Faure Karine, Fujimoto Junichi, Shimabukuro David W, Ajayi Temitayo, Shime Nobuaki, Moriyama Kiyoshi, Spack Edward G, Wiener-Kronish Jeanine P, Sawa Teiji
Department of Anesthesia and Perioperative Care, University of California, San Francisco, CA94143-0542, U,S,A.
J Immune Based Ther Vaccines. 2003 Aug 13;1(1):2. doi: 10.1186/1476-8518-1-2.
The effects of the murine monoclonal anti-PcrV antibody Mab166 on acute lung injury induced by Pseudomonas aeruginosa were analyzed in a rat model. METHODS: Lung injury was induced by the instillation of P. aeruginosa strain PA103 directly into the left lungs of anesthetized rats. One hour after the bacterial instillation, rabbit polyclonal anti-PcrV IgG, murine monoclonal anti-PcrV IgG Mab166 or Mab166 Fab-fragments were administered intratracheally directly into the lungs. The degree of alveolar epithelial injury, amount of lung edema, decrease in oxygenation and extent of lung inflammation by histology were evaluated as independent parameters of acute lung injury. RESULTS: These parameters improved in rats that had received intratracheal instillation of either rabbit polyclonal anti-PcrV IgG, murine monoclonal anti-PcrV IgG Mab166 or Mab166 Fab-fragments in comparison with the control group. CONCLUSION: Mab166 and its Fab fragments have potential as adjuvant therapy for acute lung injury due to P. aeruginosa pneumonia.
在大鼠模型中分析了鼠单克隆抗PcrV抗体Mab166对铜绿假单胞菌诱导的急性肺损伤的影响。方法：通过将铜绿假单胞菌菌株PA103直接注入麻醉大鼠的左肺来诱导肺损伤。细菌注入1小时后，将兔多克隆抗PcrV IgG、鼠单克隆抗PcrV IgG Mab166或Mab166 Fab片段经气管内直接注入肺中。将肺泡上皮损伤程度、肺水肿量、氧合降低以及组织学上的肺炎症程度作为急性肺损伤的独立参数进行评估。结果：与对照组相比，接受气管内注入兔多克隆抗PcrV IgG、鼠单克隆抗PcrV IgG Mab166或Mab166 Fab片段的大鼠的这些参数有所改善。结论：Mab166及其Fab片段有潜力作为铜绿假单胞菌肺炎所致急性肺损伤的辅助治疗药物。
