Rodriguez Sylvain, Compagnon Vincent, Crouch Nicholas P, St-Pierre Benoit, De Luca Vincenzo
Service de l'environnement et de l'énergie, Les Croisettes, CP 33 1066 Epalinges, Switzerland.
Phytochemistry. 2003 Sep;64(2):401-9. doi: 10.1016/s0031-9422(03)00269-3.
Methyl jasmonate, a chemical inducer of secondary metabolism, was shown to promote tabersonine 2 biosynthesis in hairy root cultures of Catharanthus roseus. Tabersonine 6,7-epoxidase activity was detected in total protein extract of jasmonate-induced hairy root cultures using labeled 14C-tabersonine 2. This enzyme converted tabersonine 2 to lochnericine 3 by selective epoxidation at positions 6 and 7 via a reaction dependent on NADPH and molecular oxygen. Carbon monoxide, clotrimazole, miconazole, and cytochrome C were shown to be strong inhibitors of the enzyme. The activity was found in microsomes, indicating that tabersonine 6,7-epoxidase was a cytochrome P-450-dependent monooxygenase.
茉莉酸甲酯是一种次生代谢的化学诱导剂，已证明它能促进长春花毛状根培养物中利血胺2的生物合成。使用标记的14C-利血胺2在茉莉酸诱导的毛状根培养物的总蛋白提取物中检测到利血胺6,7-环氧化酶活性。该酶通过依赖于NADPH和分子氧的反应，在6位和7位进行选择性环氧化，将利血胺2转化为洛柯宁碱3。一氧化碳、克霉唑、咪康唑和细胞色素C被证明是该酶的强抑制剂。该活性存在于微粒体中，表明利血胺6,7-环氧化酶是一种细胞色素P-450依赖性单加氧酶。
