Furuya T, Sakamoto K, Iida K, Asada Y, Yoshikawa T, Sakai S, Aimi N
School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Phytochemistry. 1992 Sep;31(9):3065-8. doi: 10.1016/0031-9422(92)83447-7.
To investigate the reactions involved in the biosynthesis of vindoline from tabersonine, the bioconversion products formed when the latter compound was fed to cell suspension cultures of Catharanthus roseus were isolated and characterized. Two biotransformation products of tabersonine were isolated and shown to be lochnericine, which is formed by epoxidation of tabersonine at positions 14, 15, and lochnerinine, the 11-methoxylation product of lochnericine. The bioconversion ratio of the main biotransformation product, lochnericine, reached a value of 80.6% within three days.
为了研究从利血平宁生物合成长春花碱过程中涉及的反应，将后一种化合物加入长春花细胞悬浮培养物中时形成的生物转化产物进行了分离和表征。分离出了利血平宁的两种生物转化产物，分别鉴定为洛柯碱，它是由利血平宁在14、15位环氧化形成的，以及洛柯宁碱，它是洛柯碱的11 - 甲氧基化产物。主要生物转化产物洛柯碱的生物转化率在三天内达到了80.6%。
