利用同工酶和随机扩增多态性DNA分析对巴西人体十二指肠贾第虫分离株进行分子特征分析。

Molecular characterization of Brazilian human Giardia duodenalis isolates using isoenzyme and random amplified polymorphic DNA analysis.

作者信息

Rocha Míriam O, Gomes Maria A, Costa Adriana O, Furst Cinthia, Silva Edward F

机构信息

School of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

出版信息

Diagn Microbiol Infect Dis. 2003 Aug;46(4):273-8. doi: 10.1016/s0732-8893(03)00083-x.

DOI:10.1016/s0732-8893(03)00083-x

PMID:12944019

Abstract

Isoenzymes and RAPD (random amplified polymorphic DNA) analysis were used to characterize three Brazilian human isolates of Giardia duodenalis and its clones. The Portland-1 strain (ATCC 30888) was included in the study as a reference pattern. Both methods divided the isolates into two main groups, one represented by the Portland-1 strain, the other constituted by the Brazilian isolates, which, in turn, were divided into 2 subgroups. The dendogram constructed with the RAPD data, using seven primers, revealed a great heterogeneity between Brazilian isolates and the Portland-1 strain. There was no relationship to the clinical characteristics of the isolates. Although a lot of similarity has been observed among Brazilian isolates and its clones, individual polymorphism was detected, which could be related to the clonal reproduction of this protozoan.

摘要

采用同工酶和随机扩增多态性DNA（RAPD）分析对三株巴西人源十二指肠贾第鞭毛虫分离株及其克隆进行特征分析。研究中纳入波特兰-1株（ATCC 30888）作为参考模式。两种方法均将分离株分为两个主要组，一组以波特兰-1株为代表，另一组由巴西分离株组成，而巴西分离株又分为2个亚组。利用7种引物构建的基于RAPD数据的树状图显示，巴西分离株与波特兰-1株之间存在很大的异质性。这与分离株的临床特征无关。尽管在巴西分离株及其克隆之间观察到很多相似性，但仍检测到个体多态性，这可能与这种原生动物的克隆繁殖有关。