贾第虫DNA的随机扩增多态性DNA（RAPD）分析及其与同工酶数据的相关性。

RAPD (random amplified polymorphic DNA) analysis of Giardia DNA and correlation with isoenzyme data.

作者信息

Morgan U M, Constantine C C, Greene W K, Thompson R C

机构信息

Institute for Molecular Genetics and Animal Disease, School of Veterinary Studies, Murdoch University, Western Australia.

出版信息

Trans R Soc Trop Med Hyg. 1993 Nov-Dec;87(6):702-5. doi: 10.1016/0035-9203(93)90303-8.

DOI:10.1016/0035-9203(93)90303-8

PMID:8296385

Abstract

Fourteen Giardia duodenalis isolates were examined using the RAPD (random amplified polymorphic deoxyribonucleic acid) technique. Simple reproducible polymorphisms were generated using 3 different RAPD primers. The results generated by each primer were very similar and were significantly correlated with each other. These data were then compared to existing isoenzyme electrophoresis data on the same isolates. The RAPD data divided the isolates into 10 groupings or rapdemes while the isoenzyme data divided them into 10 similar zymodemes. Both methods grouped 4 isolates (BAH42, BAH44c9, BAH12c9 and BAH39c7), which comprised a phenotypically heterogeneous assemblage with respect to growth rate and metabolism, into similar groupings. The 2 methods were significantly correlated (P < 0.001). It will therefore be possible to use RAPD for the characterization of isolates of Giardia, and other parasites such as Cryptosporidium, which are refractory to cultivation in vitro.

摘要

使用随机扩增多态性脱氧核糖核酸（RAPD）技术对14株十二指肠贾第虫分离株进行了检测。使用3种不同的RAPD引物产生了简单可重复的多态性。每个引物产生的结果非常相似，且彼此之间显著相关。然后将这些数据与同一分离株现有的同工酶电泳数据进行比较。RAPD数据将分离株分为10个分组或RAP型，而同工酶数据将它们分为10个相似的酶型。两种方法都将4株分离株（BAH42、BAH44c9、BAH12c9和BAH39c7）归为相似分组，这4株分离株在生长速率和代谢方面构成了一个表型异质的组合。两种方法显著相关（P < 0.001）。因此，有可能使用RAPD对贾第虫以及其他难以在体外培养的寄生虫（如隐孢子虫）的分离株进行特征描述。