对来自与英国和爱尔兰的暴发及散发病例相关的肠致病性大肠杆菌的intimin基因进行分型。
Subtyping intimin genes from enteropathogenic Escherichia coli associated with outbreaks and sporadic cases in the United Kingdom and Eire.
作者信息
Jenkins C, Lawson A J, Cheasty T, Willshaw G A, Wright P, Dougan G, Frankel G, Smith H R
机构信息
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Specialist and Reference Microbiology Division, Health Protection Agency, 61 Colindale Avenue, NW9 5HT, London, UK.
出版信息
Mol Cell Probes. 2003 Aug;17(4):149-56. doi: 10.1016/s0890-8508(03)00046-x.
PCR-RFLP methods for subtyping the intimin gene from strains of typical and atypical enteropathogenic Escherichia coli (EPEC) and Verocytotoxin-producing E. coli (VTEC) were compared. A novel HhaI PCR-RFLP method was developed that was rapid, easy to use and amplified an 1852 bp fragment of the intimin gene from all isolates examined. This method was used to investigate the intimin sub-types of EPEC strains associated with 14 outbreaks of diarrhoeal disease between 1967 and 2001, and 20 sporadic cases between January and December 2000, in the UK and Eire. In this study, genes encoding alpha, beta, gamma, delta and zeta-intimin were detected in the EPEC strains associated with outbreaks and beta, gamma, epsilon, theta and zeta-intimin genes were identified in isolates from sporadic cases. The beta-intimin gene was the most frequently detected sub-type in both the outbreak and sporadic strains.
对用于对典型和非典型肠致病性大肠杆菌（EPEC）及产志贺毒素大肠杆菌（VTEC）菌株的intimin基因进行亚型分析的PCR-RFLP方法进行了比较。开发了一种新型的HhaI PCR-RFLP方法，该方法快速、易用，并且能从所有检测的分离株中扩增出intimin基因的一个1852 bp片段。该方法用于调查1967年至2001年间在英国和爱尔兰与14起腹泻病暴发相关的EPEC菌株以及2000年1月至12月期间20例散发病例的intimin亚型。在本研究中，在与暴发相关的EPEC菌株中检测到编码α、β、γ、δ和ζ-intimin的基因，在散发病例的分离株中鉴定出β、γ、ε、θ和ζ-intimin基因。β-intimin基因是在暴发菌株和散发病例菌株中最常检测到的亚型。
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