Krause Gladys, Zimmermann Sonja, Beutin Lothar
Division of Microbial Toxins, Department of Biological Safety, Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany.
Vet Microbiol. 2005 Mar 20;106(1-2):87-95. doi: 10.1016/j.vetmic.2004.11.012.
Domestic animals belonging to seven different species (cattle, sheep, dogs, cats, pigs, chicken and goats) were investigated as natural reservoirs for attaching and effacing Escherichia coli (AEEC). For this, 2165 E. coli strains from faeces of 803 animals were examined for the presence of the intimin -(eae) gene as a characteristic of AEEC strains. Ten percent of the animals were found to excrete AEEC, most frequently found in sheep (19.2%) and pigs (17.6), followed by cattle (10.4%), dogs (7.2%), cats (6.5%) and poultry (2.3%). The 97 AEEC strains from animals were grouped into 44 serotypes. Only four E. coli serotypes (O2:H8, O26:[H11], O109:[H25] and O145:[H28] were found in more than one animal host species. AEEC O26:[H11] strains were most frequently isolated (13.4%) being present in cattle, poultry, pigs and sheep. A search for virulence markers associated with enterohemorrhagic E. coli (EHEC) revealed Shiga-toxin genes in three (3.1%) AEEC strains from sheep. Bundle forming pili genes as a trait of typical enteropathogenic E. coli (EPEC) were detected in four (4.1%) strains from dogs and cats. The remaining 90 AEEC strains were classified as atypical EPEC. Typing of intimin genes revealed intimin beta being present in 51.5% of the strains, followed by intimins theta (23.7%), epsilon (6.2%), kappa (5.2%), zeta (5.2%), alpha, eta and iota (each 1.0%). Our data indicate that domestic animals and pets constitute an important natural reservoir of AEEC strains, and some of these (O26:[H11], O103:H2, O128:H2, O145:[H28] and O177:[H11]) are known to occur as pathogens in humans.
对属于七个不同物种（牛、羊、狗、猫、猪、鸡和山羊）的家畜作为紧密黏附性大肠杆菌（AEEC）的自然宿主进行了调查。为此，对来自803只动物粪便中的2165株大肠杆菌菌株检测了intimin-（eae）基因的存在情况，以此作为AEEC菌株的特征。发现10%的动物排泄AEEC，最常见于绵羊（19.2%）和猪（17.6%），其次是牛（10.4%）、狗（7.2%）、猫（6.5%）和家禽（2.3%）。从动物中分离出的97株AEEC菌株分为44个血清型。只有四种大肠杆菌血清型（O2:H8、O26:[H11]、O109:[H25]和O145:[H28]）在不止一种动物宿主物种中被发现。AEEC O26:[H11]菌株最常被分离到（13.4%），存在于牛、家禽、猪和绵羊中。对与肠出血性大肠杆菌（EHEC）相关的毒力标记物的搜索发现，来自绵羊的三株（3.1%）AEEC菌株中有志贺毒素基因。在来自狗和猫的四株（4.1%）菌株中检测到作为典型肠致病性大肠杆菌（EPEC）特征的束状菌毛基因。其余90株AEEC菌株被归类为非典型EPEC。intimin基因分型显示，51.5%的菌株存在intimin beta，其次是intimin theta（23.7%）、epsilon（6.2%）、kappa（5.2%）、zeta（5.2%）、alpha、eta和iota（各1.0%）。我们的数据表明，家畜和宠物构成了AEEC菌株的重要自然宿主，其中一些（O26:[H11]、O103:H2、O128:H2、O145:[H28]和O177:[H11]）已知可作为人类病原体出现。
