[用于植物乳杆菌8R-A3培养的营养培养基的研制]
[Development of nutrient medium for the cultivation of Lactobacillus plantarum 8R-A3 ].
作者信息
Sorokulova I B, Khil'ko T V, Osadchaia A I
机构信息
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
出版信息
Mikrobiol Z. 2003 May-Jun;65(3):39-45.
Optimal nutrient medium providing accumulation of cell biomass of Lactobacillus plantarum 8R-A3 has been developed. According to this index the proposed medium meets the nutrition requirements of bacteria under study and is comparable with the basal (MRS) medium. Dependence of growth properties of Lactobacillus plantarum 8R-A3 on concentration of the medium basal components has been studied. Maize extract (6 vol.%) and enzymolysate of fodder yeast (5 vol.%) are the most favourable sources of nitrogen nutrition. Addition of pantothenic, nicotinic acids or riboflavin to the nutrient medium increased the yield of Lactobacillus plantarum biomass.
已开发出能促进植物乳杆菌8R-A3细胞生物量积累的最佳营养培养基。据此指标，所提出的培养基满足了受试细菌的营养需求，且与基础（MRS）培养基相当。研究了植物乳杆菌8R-A3生长特性对培养基基础成分浓度的依赖性。玉米提取物（6%体积）和饲料酵母酶解物（5%体积）是最有利的氮营养来源。向营养培养基中添加泛酸、烟酸或核黄素可提高植物乳杆菌生物量的产量。
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