Tu C, Liang H, Wang G
Department of Chemistry and Chemical Engineering, Guangxi Normal University, 541004 Guilin.
Guang Pu Xue Yu Guang Pu Fen Xi. 2001 Aug;21(4):524-6.
The influence of the mixing time of Pd(II) and Rhus vernicifera laccase on the oxidation of 5,6-dibromo-2,3-dicyanohydroquinone (DDBQH2) catalyzed by laccase has been studied through spectrophotometer at pH 4.5 and (30 +/- 0.1 degree C). The activation of Pd(II) on the laccase activity is gradually converted into inhibition and ultimately inactivation with the extension of the mixing time of Pd(II) and laccase, and the rate of such conversion goes up with the increase of Pd(II) concentration. The influence of Pd(II) on the absorption spectrum of laccase suggests that it is the type I site in laccase with which Pd(II) interacts and Pd(II) may gradually and at last substitute Cu(II) from the site. The substitution may be the cause of decrease and disappearance of laccase activity.
在pH值为4.5、温度为（30±0.1）℃的条件下，通过分光光度计研究了钯（II）与漆树漆酶的混合时间对漆酶催化5,6-二溴-2,3-二氰基对苯二酚（DDBQH2）氧化的影响。随着钯（II）与漆酶混合时间的延长，钯（II）对漆酶活性的激活作用逐渐转变为抑制作用并最终导致失活，且这种转变速率随钯（II）浓度的增加而升高。钯（II）对漆酶吸收光谱的影响表明，钯（II）与漆酶中的I型位点相互作用，并且钯（II）可能会逐渐并最终从该位点取代铜（II）。这种取代可能是漆酶活性降低和消失的原因。
