Tu C, Liang H, Wang G
Department of Chemistry and Chemical Engineering, Guangxi Normal University, 541004 Guilin.
Guang Pu Xue Yu Guang Pu Fen Xi. 2000 Aug;20(4):572-4.
The influence of the mixed time of Hg2+ ion and Rhus vernicifera laccase on the oxidation of 5,6-dibromo-2,3-dicyanohydroquinone (DDBQH2) catalyzed by laccase has been studied at pH 4.4 and 30 +/- 0.2 degrees C. Hg2+ ion can activate the catalytic activity of laccase when the mixed time is short. With the extension of the mixed time, the activation is gradually converted into inhibition. The influence of Hg2+ ion on the absorption spectrum of laccase suggests that it may be the substitution that Hg2+ ion displaces Cu(II) from the type 1 site in laccase causing the inhibition of laccase activity.
在pH值为4.4、温度为30±0.2℃的条件下，研究了Hg2+离子与漆树漆酶的混合时间对漆酶催化5,6-二溴-2,3-二氰基对苯二酚（DDBQH2）氧化反应的影响。当混合时间较短时，Hg2+离子可激活漆酶的催化活性。随着混合时间的延长，这种激活作用逐渐转变为抑制作用。Hg2+离子对漆酶吸收光谱的影响表明，可能是Hg2+离子从漆酶的1型位点取代了Cu(II)，从而导致漆酶活性受到抑制。
