Suneetha Lavanya M, Singh Surya S, Vani Meher, Vardhini Deena, Scollard David, Archelos Juan J, Srinivasulu M, Suneetha Sujai
LEPRA India-Blue Peter Research Centre, Hyderabad, India.
Neurochem Res. 2003 Sep;28(9):1393-9. doi: 10.1023/a:1024904717612.
We have previously shown that a major phosphorylated 25-kDa glycoprotein of the human peripheral nerve binds to Mycobacterium leprae. In the present study, we confirm that the 25-kDa glycoprotein of the human peripheral nerve is myelin P zero (P0) by immunoprecipitation and Western blot experiments using monoclonal antibodies to myelin P0. Immunohistochemical studies on human nerve using these antibodies to myelin P0 exhibited a strong immunoreactivity to the myelin and Schwann cells. Myelin P0 is a peripheral nerve specific protein; therefore it could likely be one of the key target molecules for M. leprae binding/internalization or even contact-dependent demyelination. This finding of M. leprae binding to myelin P0 adds to the present understanding on neural predilection of M. leprae.
我们之前已经表明，人类外周神经中一种主要的磷酸化25 kDa糖蛋白可与麻风分枝杆菌结合。在本研究中，我们通过使用针对髓鞘P0的单克隆抗体进行免疫沉淀和蛋白质印迹实验，证实人类外周神经的25 kDa糖蛋白是髓鞘P0。使用这些针对髓鞘P0的抗体对人类神经进行免疫组织化学研究，结果显示对髓鞘和施万细胞有强烈的免疫反应性。髓鞘P0是一种外周神经特异性蛋白；因此，它很可能是麻风分枝杆菌结合/内化甚至接触依赖性脱髓鞘的关键靶分子之一。麻风分枝杆菌与髓鞘P0结合的这一发现增进了我们目前对麻风分枝杆菌神经偏好性的理解。
