Nakata Naohiro, Ishida Akihiko, Tani Hirofumi, Kamidate Tamio
Graduate School of Engineering, Hokkaido University, Kita-13, Nishi-8, Kita-ku, Sapporo 060-8628, Japan.
Anal Sci. 2003 Aug;19(8):1183-5. doi: 10.2116/analsci.19.1183.
Cationic liposomes composed of two components, diethylaminoethyl-carbamoyl cholesterol and phosphatidylcholine, were applied to an enhancer for a firefly bioluminescent (BL) assay of bacterial ATP in the presence of an ATP extractant. Trichloroacetic acid (TCA), which inhibits the activity of luciferase, was used as an ATP extractant. Cationic liposomes enhanced the BL intensity as long as luciferase was active. The detection limits for cell numbers of Escherichia coli extracts in the presence of cationic liposomes and in water alone were 199 and 897 colony forming units ml(-1), respectively. The sensitivity for bacterial ATP in the presence of cationic liposomes was improved by a factor of 2.5 times compared to that in the presence of diethylaminoethyl-dextran.
由二乙氨基乙基 - 氨基甲酰胆固醇和磷脂酰胆碱两种成分组成的阳离子脂质体，在存在ATP提取剂的情况下，被用作萤火虫生物发光（BL）法检测细菌ATP的增强剂。抑制荧光素酶活性的三氯乙酸（TCA）被用作ATP提取剂。只要荧光素酶有活性，阳离子脂质体就能增强生物发光强度。在存在阳离子脂质体和仅在水中的情况下，大肠杆菌提取物细胞数量的检测限分别为199和897菌落形成单位ml(-1)。与存在二乙氨基乙基 - 葡聚糖的情况相比，在存在阳离子脂质体的情况下，对细菌ATP的灵敏度提高了2.5倍。
