Kamidate T, Kinkou T, Watanabe H
Faculty of Engineering, Hokkaido University, Sapporo, Japan.
Anal Biochem. 1997 Jan 1;244(1):62-6. doi: 10.1006/abio.1996.9832.
Cationic liposomes containing stearylamine enhanced the intensity of maximum light emission from the firefly luciferin-luciferase reaction with ATP in the range of 1.0 pM up to 5.5 nM. In contrast, the bioluminescent intensities in the presence of anionic and zwitterionic liposomes were about the same as those in water alone. The detection limit for ATP in the presence of cationic liposomes was 1.0 pM in aqueous ATP standard solutions. The sensitivity for ATP was improved by a factor of five times compared to that in water alone. The method was applied to the determination of ATP in Escherichia coli extracts. The sensitivity threshold was presumed to be 340 cells ml-1.
含有硬脂胺的阳离子脂质体增强了萤火虫荧光素-荧光素酶与ATP反应的最大发光强度，范围为1.0皮摩尔至5.5纳摩尔。相比之下，阴离子脂质体和两性离子脂质体存在时的生物发光强度与仅在水中时大致相同。在水性ATP标准溶液中，阳离子脂质体存在时ATP的检测限为1.0皮摩尔。与仅在水中相比，对ATP的灵敏度提高了五倍。该方法应用于大肠杆菌提取物中ATP的测定。灵敏度阈值推测为每毫升340个细胞。
