Gagliardi Luigi, De Orsi Daniela, Chimenti Paola, Porra' Rita, Tonelli Domenica
Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanitá, Rome, Italy.
Anal Sci. 2003 Aug;19(8):1195-7. doi: 10.2116/analsci.19.1195.
A simple HPLC method for the determination of imidazole antimycotics in cosmetic antidandruff formulations has been developed. HPLC was carried out on a Discovery RP-Amide C16 column and spectrophotometric detection was performed at 220 nm. The initial mobile phase was a mixture of acetonitrile and aqueous 10(-3) M NaClO4 (pH 3.0) in the ratio of 15:85 (v/v); then a linear gradient up to 46% acetonitrile in 70 min, and up to 50% in 80 min. The extraction procedure has been validated by analyzing samples of shampoo and lotion spiked with 1% of the active principles. The recoveries were greater than 95% and the reproducibility was within 3%.
已开发出一种用于测定化妆品去屑配方中咪唑类抗真菌剂的简单高效液相色谱法。高效液相色谱在Discovery RP-Amide C16柱上进行，分光光度检测在220 nm波长下进行。初始流动相为乙腈与10(-3) M高氯酸钠水溶液（pH 3.0）按15:85（v/v）比例混合；然后在70分钟内线性梯度至46%乙腈，80分钟内至50%乙腈。通过分析添加1%活性成分的洗发水和乳液样品验证了提取程序。回收率大于95%，重现性在3%以内。
