高效液相色谱法测定化妆品中的12种防晒剂

[Determination of 12 sunscreen agents in cosmetics by high performance liquid chromatography].

作者信息

He Qiaosang, Xu Na, Li Jing, Liao Shangfu

机构信息

Zhejiang Test Academy of Quality and Technical Supervision, Hangzhou 310013, China.

出版信息

Se Pu. 2011 Aug;29(8):762-7.

PMID:22128740

Abstract

A comprehensive analytical method based on high performance liquid chromatography (HPLC) has been developed for the determination of 12 sunscreen agents in cosmetics. The cosmetic samples were extracted by methanol. The target compounds were separated on an SB-C8 column (250 mm x 4.6 mm, 5 microm) in gradient elution mode using methanol and 0.1% (v/v) formic acid aqueous solution as mobile phases. The detective wavelength was 311 nm. The linear plots were obtained between 1.0 and 500 mg/L with good correlation coefficients larger than 0.999 5. The limits of detection (LODs) for this method were in the range of 0.002 -0.1 mg/L. The spiked recoveries of commercial cosmetics were in the range of 97.4% - 107.5% with the relative standard deviations of 1.54% -4.98%. The results indicated that the developed method is simple, rapid, accurate and suitable for the determination of 12 sunscreen agents in cosmetics samples.

摘要

已开发出一种基于高效液相色谱（HPLC）的综合分析方法，用于测定化妆品中的12种防晒剂。化妆品样品用甲醇提取。目标化合物在SB-C8柱（250 mm×4.6 mm，5μm）上以梯度洗脱模式分离，使用甲醇和0.1%（v/v）甲酸水溶液作为流动相。检测波长为311 nm。在1.0至500 mg/L之间获得线性图，相关系数大于0.999 5。该方法的检测限（LOD）在0.002 - 0.1 mg/L范围内。市售化妆品的加标回收率在97.4% - 107.5%范围内，相对标准偏差为1.54% - 4.98%。结果表明，所开发的方法简单、快速、准确，适用于化妆品样品中12种防晒剂的测定。