Liquori Giuseppa E, Scillitani Giovanni, Mastrodonato Maria, Ferri Domenico
Department of Zoology, Laboratory of Histology and Comparative Anatomy, University of Bari, Bari, Italy.
Histochem J. 2002 Oct;34(10):517-24. doi: 10.1023/a:1024766124211.
The secretory cells of the oesophagogastric tract of the Eurasian toad, Bufo viridis, were examined using standard histochemical methods and lectin histochemistry. Two goblet cell types were found in the oesophageal epithelium, differing in their morphology and the histochemical features of the secretory granules. These contained mainly acidic glycoconjugates, both sulphated and carboxylated, and a small amount of pepsinogen. Type I goblet cells contained stable class-III mucosubstances, which were absent in Type II. No pluricellular oesophageal glands were found. The oesophagogastric junction had a superficial epithelium similar to that of the oesophageal epithelium, with alveolar pluricellular glands, secreting stable class-III mucins, and few oxynticopeptic cells. The gastric mucosa presented secretory cells both in the surface epithelium and in the gastric glands. Superficial and foveolar cells produced neutral mucins with Gal(beta)1,3GalNAc residues. Neck cells, oxynticopeptic cells and endocrine cells were found in the gastric glands. Neck cells produced stable class-III mucosubstances. A functional gradient was observed in the oxynticopeptic cells from the oral to the aboral fundus, with a decrease in pepsinogen secretion towards the aboral fundus and a possible increase in HCl secretion. In the pyloric mucosa, the oxynticopeptic cells disappeared and the glands produced only neutral mucins, without stable class-III mucosubstances.
利用标准组织化学方法和凝集素组织化学技术，对绿蟾蜍（Bufo viridis）食管胃道的分泌细胞进行了研究。在食管上皮中发现了两种杯状细胞类型，它们在形态和分泌颗粒的组织化学特征上有所不同。这些细胞主要含有酸性糖缀合物，包括硫酸化和羧化的，以及少量胃蛋白酶原。I型杯状细胞含有稳定的III类黏液物质，II型杯状细胞中则没有。未发现多细胞食管腺。食管胃交界处有一层与食管上皮相似的表层上皮，有分泌稳定III类黏蛋白的肺泡状多细胞腺，以及少量泌酸胃酶细胞。胃黏膜在表面上皮和胃腺中均有分泌细胞。表层细胞和小凹细胞产生带有Gal(β)1,3GalNAc残基的中性黏液。胃腺中发现了颈部细胞、泌酸胃酶细胞和内分泌细胞。颈部细胞产生稳定的III类黏液物质。在泌酸胃酶细胞中观察到从口腔端到肛门端胃底存在功能梯度，向肛门端胃底方向胃蛋白酶原分泌减少，盐酸分泌可能增加。在幽门黏膜中，泌酸胃酶细胞消失，腺体仅产生中性黏液，没有稳定的III类黏液物质。
