MacCoss Rachel N, Brennan Paul E, Ley Steven V
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK CB2 1EW.
Org Biomol Chem. 2003 Jun 21;1(12):2029-31. doi: 10.1039/b303951j.
Methods to allow the clean preparation of oligosaccharides were investigated using techniques that do not require conventional column chromatography or an aqueous work-up. The route was designed to provide rapid access to oligosaccharides and is suitable for automation and parallel library formation. The research has focused on the glycosidations of a range of glycosyl acceptors with various selenophenyl glycosyl donors using iodine as an activator in the presence of DTBMP, a hindered organic base. Hydroxyl-containing contaminants were removed by scavenging with polymer-supported tosyl chloride.
研究了使用无需常规柱色谱或水相后处理的技术来实现寡糖的纯净制备的方法。该路线旨在快速获得寡糖，适用于自动化和并行文库的构建。研究重点在于在受阻有机碱DTBMP存在下，以碘作为活化剂，使一系列糖基受体与各种硒苯基糖基供体进行糖基化反应。含羟基的污染物通过用聚合物负载的对甲苯磺酰氯清除来去除。
