Kato H, Kato N, Watanabe K, Ueno K
Institute of Anaerobic Bacteriology, Gifu University School of Medicine.
Kansenshogaku Zasshi. 1992 Nov;66(11):1524-31. doi: 10.11150/kansenshogakuzasshi1970.66.1524.
Western blotting using antisera against each of reference ten serogroups was evaluated as a typing system for Clostridium difficile. A total of 164 isolates of C. difficile (114 epidemiologically unrelated and 50 isolates from a hospital outbreak in New York) were tested. Blotting patterns for the ten reference strains showed serogroup-specific bands located in the 30-60 kDa when each homologous antiserum was used. At greater than 60 kDa, variations in each serogroups were observed; these variations were used for subserogrouping the isolates. Serogroup A, G, H, and K were most frequently recovered in the group of epidemiologically unrelated isolates. Subserogroup G-1 strains of serogroup G was isolated from 28 of 36 patients (78%) of the hospital outbreak. The result suggested that the subserogroup G-1 strain was the major cause of infection in the hospital outbreak. A total of 46 of the 114 unrelated isolates (40.4%) and 9 of 50 outbreak isolates (18%) did not react with any of reference antisera and classified as nontypable. The western blotting was found to be useful not only as an epidemiological tool but as a typing system for C. difficile.
使用针对十种参考血清群的抗血清进行蛋白质免疫印迹法被评估为艰难梭菌的一种分型系统。共检测了164株艰难梭菌分离株（114株来自流行病学上无关联的样本，50株来自纽约一家医院爆发疫情的样本）。当使用每种同源抗血清时，十种参考菌株的印迹模式显示在30 - 60 kDa处有血清群特异性条带。在大于60 kDa处，观察到每个血清群的变异；这些变异被用于对分离株进行亚血清群分类。血清群A、G、H和K在流行病学上无关联的分离株组中最常被检出。血清群G的G - 1亚群菌株从医院爆发疫情的36名患者中的28名（78%）分离得到。结果表明，G - 1亚群菌株是医院爆发感染的主要原因。114株无关联分离株中有46株（40.4%）以及50株爆发疫情分离株中有9株（18%）与任何参考抗血清均无反应，被归类为不可分型。蛋白质免疫印迹法不仅被发现可作为一种流行病学工具，而且可作为艰难梭菌的一种分型系统。
