用10种不同抗血清通过蛋白质免疫印迹法对艰难梭菌进行分型。
Typing of Clostridium difficile by western immunoblotting with 10 different antisera.
作者信息
Kato H, Cavallaro J J, Kato N, Bartley S L, Killgore G E, Watanabe K, Ueno K
机构信息
Nosocomial Pathogens Laboratory Branch, Centers for Disease Control, Atlanta, Georgia 30333.
出版信息
J Clin Microbiol. 1993 Feb;31(2):413-5. doi: 10.1128/jcm.31.2.413-415.1993.
Abstract
Western blotting (immunoblotting) with antisera against each of 10 reference serogroups was evaluated as a means of typing Clostridium difficile. A total of 164 clinical isolates of C. difficile were tested. Variations in band profiles in each serogroup were used to type isolates into subserogroups. This technique was useful for an epidemiological investigation.
摘要
使用针对10个参考血清型的抗血清进行蛋白质印迹法（免疫印迹法），以此作为艰难梭菌分型的一种手段进行了评估。总共对164株艰难梭菌临床分离株进行了检测。利用每个血清型中条带图谱的差异将分离株进一步分为亚血清型。这项技术对流行病学调查很有用。
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