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用10种不同抗血清通过蛋白质免疫印迹法对艰难梭菌进行分型。

Typing of Clostridium difficile by western immunoblotting with 10 different antisera.

作者信息

Kato H, Cavallaro J J, Kato N, Bartley S L, Killgore G E, Watanabe K, Ueno K

机构信息

Nosocomial Pathogens Laboratory Branch, Centers for Disease Control, Atlanta, Georgia 30333.

出版信息

J Clin Microbiol. 1993 Feb;31(2):413-5. doi: 10.1128/jcm.31.2.413-415.1993.

DOI:10.1128/jcm.31.2.413-415.1993
PMID:8432828
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262776/
Abstract

Western blotting (immunoblotting) with antisera against each of 10 reference serogroups was evaluated as a means of typing Clostridium difficile. A total of 164 clinical isolates of C. difficile were tested. Variations in band profiles in each serogroup were used to type isolates into subserogroups. This technique was useful for an epidemiological investigation.

摘要

使用针对10个参考血清型的抗血清进行蛋白质印迹法（免疫印迹法），以此作为艰难梭菌分型的一种手段进行了评估。总共对164株艰难梭菌临床分离株进行了检测。利用每个血清型中条带图谱的差异将分离株进一步分为亚血清型。这项技术对流行病学调查很有用。

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本文引用的文献

1
Immunochemical fingerprinting of Clostridium difficile strains isolated from an outbreak of antibiotic-associated colitis and diarrhoea.从一起抗生素相关性结肠炎和腹泻暴发中分离出的艰难梭菌菌株的免疫化学指纹图谱分析
J Med Microbiol. 1984 Jun;17(3):317-24. doi: 10.1099/00222615-17-3-317.
2
Polyacrylamide gel electrophoresis patterns produced by Clostridium difficile.艰难梭菌产生的聚丙烯酰胺凝胶电泳图谱。
Rev Infect Dis. 1984 Mar-Apr;6 Suppl 1:S229-34. doi: 10.1093/clinids/6.supplement_1.s229.
3
Serogrouping of Clostridium difficile strains by slide agglutination.
Identification of toxin A-negative, toxin B-positive Clostridium difficile by PCR.通过聚合酶链反应鉴定毒素A阴性、毒素B阳性的艰难梭菌。
J Clin Microbiol. 1998 Aug;36(8):2178-82. doi: 10.1128/JCM.36.8.2178-2182.1998.
4
Relapses or reinfections: analysis of a case of Clostridium difficile-associated colitis by two typing systems.复发或再感染：通过两种分型系统对一例艰难梭菌相关性结肠炎病例的分析
Curr Microbiol. 1996 Oct;33(4):220-3. doi: 10.1007/s002849900103.
5
Use of an enzyme-linked immunoassay for Clostridium difficile serogrouping.酶联免疫分析法用于艰难梭菌血清分型
J Clin Microbiol. 1993 Sep;31(9):2526-8. doi: 10.1128/jcm.31.9.2526-2528.1993.
6
Investigation of an outbreak of Clostridium difficile infection in a general hospital by numerical analysis of protein patterns by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对蛋白质模式进行数值分析，调查一家综合医院艰难梭菌感染的暴发情况。
J Clin Microbiol. 1994 Mar;32(3):759-65. doi: 10.1128/jcm.32.3.759-765.1994.
7
Identification of outbreak-associated and other strains of Clostridium difficile by numerical analysis of SDS-PAGE protein patterns.通过SDS-PAGE蛋白图谱的数值分析鉴定与暴发相关的艰难梭菌菌株及其他菌株。
Epidemiol Infect. 1994 Aug;113(1):1-12. doi: 10.1017/s0950268800051402.
8
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9
Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing.使用随机引物PCR对艰难梭菌进行分型，并将结果与免疫印迹分型结果进行比较。
J Clin Microbiol. 1994 Jun;32(6):1591-3. doi: 10.1128/jcm.32.6.1591-1593.1994.
通过玻片凝集法对艰难梭菌菌株进行血清分型。
J Clin Microbiol. 1985 Mar;21(3):323-7. doi: 10.1128/jcm.21.3.323-327.1985.
4
Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile.艰难梭菌血清分型与聚丙烯酰胺凝胶电泳分型的比较
J Clin Microbiol. 1986 Dec;24(6):991-4. doi: 10.1128/jcm.24.6.991-994.1986.
5
Immunoblots and plasmid fingerprints compared with serotyping and polyacrylamide gel electrophoresis for typing Clostridium difficile.免疫印迹法和质粒指纹图谱与血清分型及聚丙烯酰胺凝胶电泳用于艰难梭菌分型的比较。
J Clin Microbiol. 1988 Jan;26(1):41-6. doi: 10.1128/jcm.26.1.41-46.1988.
6
Characterization of a nosocomial Clostridium difficile outbreak by using plasmid profile typing and clindamycin susceptibility testing.运用质粒图谱分型和克林霉素药敏试验对医院内艰难梭菌暴发进行特征分析。
J Infect Dis. 1988 Oct;158(4):731-6. doi: 10.1093/infdis/158.4.731.
7
Acquisition of Clostridium difficile from the hospital environment.艰难梭菌从医院环境中的获得。
Am J Epidemiol. 1988 Jun;127(6):1289-94. doi: 10.1093/oxfordjournals.aje.a114921.
8
Serotyping of Clostridium difficile.艰难梭菌的血清分型
J Clin Microbiol. 1988 Mar;26(3):426-8. doi: 10.1128/jcm.26.3.426-428.1988.
9
Detection of specific antigens for ten serogroups of Clostridium difficile.艰难梭菌十个血清型特异性抗原的检测
Mol Cell Probes. 1990 Feb;4(1):1-10. doi: 10.1016/0890-8508(90)90034-w.
10
Nosocomial Clostridium difficile colonisation and disease.医院内艰难梭菌定植与疾病
Lancet. 1990 Jul 14;336(8707):97-100. doi: 10.1016/0140-6736(90)91605-a.