[高脂血症患者血清高密度脂蛋白亚类]
[Subclasses of serum HDL in hyperlipidemia].
作者信息
Xu Yanhua, Fu Mingde, Liu Bingwen, Xu Yongxia, Yao Jia, Zhang Xuemei
机构信息
Apolipoprotein Research Laboratory, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):238-41.
OBJECTIVE
To detect the change of composition and ratio of serum HDL subclasses and explore the relationship between these changes and the plasma lipid level in patients with hyperlipidemia.
METHODS
The subclasses of serum HDL in 168 patients with hyperlipidemia were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method.
RESULTS
Compared to the healthy controls, the pre-beta 1 HDL, pre-beta 2 HDL, HDL3c, HDL3b, and HDL3a increased significantly (P < 0.01, P < 0.01, P < 0.05, P < 0.01 and P < 0.01), while the HDL2a and HDL2b decreased significantly (P < 0.01) in the patients with hyperlipidemia. The pre-beta 1 HDL and HDL3b were significantly higher (P < 0.01) while the HDL2a and HDL2b were significantly lower (P < 0.01) in men than in women with hyperlipidemia. The pre-beta 1 HDL was significantly higher (P < 0.05) while the HDL2b was significantly lower (P < 0.05) in the male control group than in the female control group. In the patients with hyperlipidemia, the serum TG was positively correlated with pre-beta 1 HDL, pre-beta 2 HDL, HDL3b, and HDL3a (r = 0.583, 0.196, 0.130, and 0.370 respectively) and negatively correlated with HDL2a and HDL2b (r = -0.293 and -0.456 respectively); The serum TC was positively correlated with pre-beta 1 HDL and HDL3b (r = 0.348 and 0.219); The serum LDL-C was positively correlated with HDL3b (r = 0.187); The serum HDL-C was negatively correlated with pre-beta 1 HDL and HDL3a (r = -0.354 and -0.292 respectively) and positively correlated with HDL2a and HDL2b (r = 0.254 and 0.256); The TG/HDL-C ratio was positively correlated with pre-beta 1 HDL, pre-beta 2 HDL, HDL3b, and HDL3a (r = 0.593, 0.225, 0.123, and 0.394) and negatively correlated with HDL2a and HDL2b (r = -0.317 and -0.459).
CONCLUSION
The particle of HDL in the patients with hyperlipidemia shows a general shift towards smaller size, which indicates that the maturation of HDL could be abnormal in patients with hyperlipidemia. The change of subclasses of HDL in the patients with hyperlipidemia might be associated with coronary artery diseases.
目的
检测高脂血症患者血清高密度脂蛋白（HDL）亚类的组成及比例变化，探讨其变化与血脂水平的关系。
方法
采用二维凝胶电泳结合免疫检测法测定168例高脂血症患者血清HDL亚类。
结果
与健康对照组相比，高脂血症患者前β1 HDL、前β2 HDL、HDL3c、HDL3b和HDL3a显著升高（P<0.01、P<0.01、P<0.05、P<0.01和P<0.01），而HDL2a和HDL2b显著降低（P<0.01）。高脂血症男性患者前β1 HDL和HDL3b显著高于女性（P<0.01），HDL2a和HDL2b显著低于女性（P<0.01）。男性对照组前β1 HDL显著高于女性对照组（P<0.05），HDL2b显著低于女性对照组（P<0.05）。高脂血症患者血清甘油三酯（TG）与前β1 HDL、前β2 HDL、HDL3b和HDL3a呈正相关（r分别为0.583、0.196、0.130和0.370），与HDL2a和HDL2b呈负相关（r分别为-0.293和-0.456）；血清总胆固醇（TC）与前β1 HDL和HDL3b呈正相关（r分别为0.348和0.219）；血清低密度脂蛋白胆固醇（LDL-C）与HDL3b呈正相关（r为0.187）；血清HDL胆固醇（HDL-C）与前β1 HDL和HDL3a呈负相关（r分别为-0.354和-0.292），与HDL2a和HDL2b呈正相关（r分别为0.254和0.256）；TG/HDL-C比值与前β1 HDL、前β2 HDL、HDL3b和HDL3a呈正相关（r分别为0.593、0.225、0.123和0.394），与HDL2a和HDL2b呈负相关（r分别为-0.317和-0.459）。
结论
高脂血症患者HDL颗粒总体向较小尺寸偏移，提示高脂血症患者HDL成熟可能异常。高脂血症患者HDL亚类变化可能与冠状动脉疾病有关。
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