高胆固醇血症和混合性高脂血症中高密度脂蛋白亚类的改变。

Alterations of high-density lipoprotein subclasses in hypercholesterolemia and combined hyperlipidemia.

作者信息

Jia Lianqun, Fu Mingde, Tian Ying, Xu Yanhua, Gou Lantu, Tian Haoming, Tian Li

机构信息

Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.

出版信息

Int J Cardiol. 2007 Sep 3;120(3):331-7. doi: 10.1016/j.ijcard.2006.10.007. Epub 2006 Dec 12.

DOI:10.1016/j.ijcard.2006.10.007

PMID:17166608

Abstract

BACKGROUND

Alterations in plasma lipid levels can influence the composition, content, and distribution of plasma lipoprotein subclasses that effect atherosclerosis risk. Hypercholesterolemia and combined hyperlipidemia are common forms of atherogenic dyslipoproteinemia. This study evaluates the alterations of high-density lipoprotein (HDL) subclasses in hypercholesterolemic and combined hyperlipidemic subjects.

METHODS

Apolipoprotein A-I contents of plasma HDL subclasses were quantitated by 2-dimensional gel electrophoresis in 242 normolipidemic subjects, 66 hypercholesterolemic subjects and 59 combined hyperlipidemic subjects.

RESULTS

Compared with the normolipidemic subjects, apolipoprotein A-I contents of small-sized pre-beta1-HDL, HDL3c, HDL3b and HDL3a were significantly higher in both hypercholesterolemic subjects (p<.01, p<.05, p<.01 and p<.05, respectively) and combined hyperlipidemic subjects (p<.01, p<.05, p<.01 and p<.01, respectively). In contrast, apolipoprotein A-I contents of large-sized HDL2a and HDL2b were significantly lower in hypercholesterolemic subjects (p<.05 and p<.01, respectively) as well as combined hyperlipidemic subjects (p<.01 and p<.01, respectively). In addition, pre-beta1-HDL increased significantly (p<.05) while HDL2a and HDL2b decreased significantly (p<.05 and p<.01, respectively) in combined hyperlipidemic group versus hypercholesterolemic subjects. With the elevation of triglyceride levels, pre-beta1-HDL, and HDL3a increased successively, however, HDL2a and HDL2b decreased successively in subjects with total cholesterol levels greater than 240 mg/dl.

CONCLUSIONS

The particle size of HDL shifted towards smaller size in hypercholesterolemic subjects, and that the shift was more prominent in combined hyperlipidemic subjects. The alternations mentioned above indicate that HDL maturation might be abnormal, and reverse cholesterol transport (RCT) might be weakened.

摘要

背景

血浆脂质水平的改变会影响血浆脂蛋白亚类的组成、含量和分布，进而影响动脉粥样硬化风险。高胆固醇血症和混合性高脂血症是致动脉粥样硬化性血脂异常的常见形式。本研究评估高胆固醇血症和混合性高脂血症患者高密度脂蛋白（HDL）亚类的变化。

方法

采用二维凝胶电泳法对242名血脂正常者、66名高胆固醇血症患者和59名混合性高脂血症患者血浆HDL亚类中的载脂蛋白A-I含量进行定量分析。

结果

与血脂正常者相比，高胆固醇血症患者（分别为p<0.01、p<0.05、p<0.01和p<0.05）和混合性高脂血症患者（分别为p<0.01、p<0.05、p<0.01和p<0.01）中，小尺寸前β1-HDL、HDL3c、HDL3b和HDL3a的载脂蛋白A-I含量均显著升高。相反，高胆固醇血症患者（分别为p<0.05和p<0.01）以及混合性高脂血症患者（分别为p<0.01和p<0.01）中，大尺寸HDL2a和HDL2b的载脂蛋白A-I含量均显著降低。此外，与高胆固醇血症患者相比，混合性高脂血症组中前β1-HDL显著升高（p<0.05），而HDL2a和HDL2b显著降低（分别为p<0.05和p<0.01）。在总胆固醇水平大于240mg/dl的受试者中，随着甘油三酯水平的升高，前β1-HDL和HDL3a依次升高，然而，HDL2a和HDL2b依次降低。