[微量法在卵巢癌体外化疗敏感性检测中的应用]

[Application of a micromethod in ovarian cancer chemosensitivity testing in vitro].

作者信息

Liu Shanling, Qian Xiaolei, Peng Zhilan, Wang He

机构信息

Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):298-9, 312.

PMID:12947719

Abstract

OBJECTIVE

To assess the efficacy of microdetermination in the ATP-bioluminescence assay (ATP-CVA) of ovarian cancer chemosensitivity testing in vitro.

METHODS

A micromethod ATP-CVA was employed in determining the chemosensitivity of 35 specimens of fresh ovarian cancer cells using Victor 2 Multilabel Counter.

RESULTS

By use of Victor 2 Multilabel Counter to determine ATP, in a range of 10(-9)-10(-5) mol/ml, a linear relationship was remarkable between the log ATP and the log luminescence. The equation was Y = 0.892X + 10.257, the correlation coefficient r was 0.998, P < 0.01. 2. The coefficient of variation obtained from ATP-CVA was 7.3%. 3. The sensitivity of the test was 92.0%, the specificity 70.0%, and the total predictive accuracy of ATP-CVA 87.1%.

CONCLUSION

With the use of Victor 2 Multilabel Counter, ATP-CVA is fast, cost-effective, and easy to perform with only small error.

摘要

目的

评估微量测定法在体外卵巢癌化疗药敏试验的ATP生物发光法（ATP-CVA）中的有效性。

方法

采用微量ATP-CVA法，使用Victor 2多功能微孔板检测仪测定35例新鲜卵巢癌细胞的化疗药敏性。

结果

使用Victor 2多功能微孔板检测仪测定ATP，在10(-9)-10(-5) mol/ml范围内，log ATP与log发光之间呈显著线性关系。方程为Y = 0.892X + 10.257，相关系数r为0.998，P < 0.01。2. ATP-CVA的变异系数为7.3%。3. 该检测的敏感性为92.0%，特异性为70.0%，ATP-CVA的总预测准确性为87.1%。