Koga H, Miyazaki Y, Kohno S, Maesaki S, Kaku M, Hara K
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Kekkaku. 1992 Dec;67(12):795-802.
The DNA probe and polymerase chain reaction (PCR) technique for detection and identification of mycobacteria were compared with the conventional smear and culture method. The results of identification by DNA probe agreed well with those of the biochemical method. Moreover, six percent of Mycobacterium avium complex (MAC) were revealed to be mycobacteria other than MAC by DNA probe. The nested PCR for detection of gene coding protein antigen b of Mycobacterium tuberculosis complex showed excellent specificity and sensitivity. Then we applied this technique to rapid detection of M. tuberculosis in 222 clinical samples. The agreement between nested PCR and the biochemical method was excellent, and 17 cases were diagnosed by only nested PCR in spite of negative results by smear and culture. These cases were unlikely to have yielded false positive results since their clinical features were compatible to tuberculosis. From these data, it was considered that the DNA probe and PCR technique were extremely useful strategies and would contribute to rapid diagnosis of mycobacterial infectious diseases.
将用于检测和鉴定分枝杆菌的DNA探针及聚合酶链反应（PCR）技术与传统涂片和培养方法进行了比较。DNA探针鉴定结果与生化方法的结果高度一致。此外，DNA探针显示6%的鸟分枝杆菌复合群（MAC）为非MAC分枝杆菌。用于检测结核分枝杆菌复合群编码蛋白抗原b基因的巢式PCR显示出极佳的特异性和敏感性。随后我们将该技术应用于222份临床样本中结核分枝杆菌的快速检测。巢式PCR与生化方法之间的一致性极佳，尽管涂片和培养结果为阴性，但仍有17例仅通过巢式PCR诊断出来。鉴于这些病例的临床特征与结核病相符，不太可能出现假阳性结果。基于这些数据，认为DNA探针和PCR技术是非常有用的策略，将有助于分枝杆菌感染性疾病的快速诊断。
