Yamazaki T, Nakamura R M
Department of Cellular Immunology, National Institute of Health, Tokyo, Japan.
Kekkaku. 1992 Jun;67(6):441-7.
The polymerase chain reaction (PCR) was used to detect mycobacterial DNA sequences in the cultured or the clinical specimens. Four oligonucleotide primers derived from the sequence of a gene coding 65-kilodalton antigen of Mycobacterium tuberculosis amplified DNA samples of all the 11 species of mycobacteria tested. Serial dilution of M. bovis BCG showed that DNA extracted from only 12 bacilli was enough for the detection by PCR method. However, mycobacteria in sputum were detected by PCR when more than 10(3) bacilli were present. The PCR method may become a useful tool for the rapid diagnosis of mycobacterial infections.
聚合酶链反应（PCR）用于检测培养的或临床标本中的分枝杆菌DNA序列。从编码结核分枝杆菌65千道尔顿抗原的基因序列中衍生出的4种寡核苷酸引物，扩增了所有11种受试分枝杆菌的DNA样本。牛分枝杆菌卡介苗的系列稀释表明，仅从12个杆菌中提取的DNA就足以通过PCR方法进行检测。然而，当痰中存在超过10³个杆菌时，可通过PCR检测到分枝杆菌。PCR方法可能成为快速诊断分枝杆菌感染的有用工具。
