Tharnprisarn W, Suwajanakorn S, Sereepapong W, Pruksananonda K, Boonyakasemsanti W, Virutamasen P, Ahnonkitpanit V, Chompurat D, Numchaisrika P
Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
J Med Assoc Thai. 2003 Jul;86(7):666-71.
To compare mouse blastocyst survival after cryopreservation with vitrification and the slow-freezing method, one-hundred and forty-eight in vitro mouse blastocysts were randomly frozen by the two methods: vitrification and conventional slow-freezing. After being thawed, the blastocysts were assessed for survival and hatching rate. The survival rates of blastocysts cryopreserved by vitrification and slow-freezing were 68.33 and 65.52 per cent (p = 0.89), whereas hatching rates were 51.22 and 44.74 per cent, respectively (p = 0.64). Therefore, vitrification of blastocyst-stage-embryos may be a useful, economic method for freezing the excess blastocysts in some centers where blastocysts are routinely transferred.
为比较玻璃化冷冻法和慢速冷冻法对小鼠囊胚冷冻保存后的存活率，将148枚体外培养的小鼠囊胚随机采用这两种方法冷冻：玻璃化冷冻法和传统慢速冷冻法。解冻后，评估囊胚的存活率和孵化率。玻璃化冷冻法和慢速冷冻法保存的囊胚存活率分别为68.33%和65.52%（p = 0.89），而孵化率分别为51.22%和44.74%（p = 0.64）。因此，对于一些常规移植囊胚的中心，囊胚期胚胎的玻璃化冷冻可能是一种有用且经济的方法，可用于冷冻多余的囊胚。
