Li Yuan, Chen Zi-jiang, Yang Hui-jun, Zhong Wan-xia, Ma Shui-ying, Li Mei
Reproductive Medical Center, Shandong Provincial Hospital, Shandong University, Jinan 250021, China.
Zhonghua Fu Chan Ke Za Zhi. 2007 Nov;42(11):753-5.
To compare the effects of vitrification with slow-freezing on the developmental ability of day 3 cleavage stage embryos.
Patients who had no less than 4 high quality embryos were included in this study. These embryos were cryopreserved using the methods of vitrification or slow-freezing. In the cryopreserved embryo transfer cycles, the embryos which were cryopreserved using one of the methods were chosen randomly. The developmental ability of embryos was compared between these two groups.
A total of 80 patients were included in this study with 160 embryos. In the group of slow-freezing, 73 (91%) embryos were survived and achieved 15 (38%) clinical pregnancies. Among these, 3 were twins and the implantation rate was 25% (18/73). In the group of vitrification, 71 (89%) embryos were survived and achieved 19 (48%) clinical pregnancies. Among these, 9 were twins and the implantation rate was 39% (28/71), which was significantly higher than the slow-freezing group (P < 0.05). Otherwise, the clinical pregnant rate and multiple pregnant rate was higher in the group of vitrification than the slow-freezing group, but had no significance.
Vitrification is more benefit for the developmental ability of the thawed embryos and more suitable for the cryopreservation of day 3 cleavage stage embryos.
比较玻璃化冷冻与慢速冷冻对第3天卵裂期胚胎发育能力的影响。
本研究纳入至少有4枚优质胚胎的患者。这些胚胎采用玻璃化冷冻或慢速冷冻方法进行冷冻保存。在冷冻胚胎移植周期中，随机选择采用其中一种方法冷冻保存的胚胎。比较两组胚胎的发育能力。
本研究共纳入80例患者，160枚胚胎。在慢速冷冻组，73枚（91%）胚胎存活，获得15例（38%）临床妊娠。其中，3例为双胎妊娠，着床率为25%（18/73）。在玻璃化冷冻组，71枚（89%）胚胎存活，获得19例（48%）临床妊娠。其中，9例为双胎妊娠，着床率为39%（28/71），显著高于慢速冷冻组（P<0.05）。此外，玻璃化冷冻组的临床妊娠率和多胎妊娠率高于慢速冷冻组，但差异无统计学意义。
玻璃化冷冻对解冻后胚胎的发育能力更有益，更适合第3天卵裂期胚胎的冷冻保存。
