Austin Maureen E, Kasturi Badrinarayanan S, Barber Matthew, Kannan Kurunthachalam, MohanKumar Puliyur S, MohanKumar Sheba M J
Neuroendocrine Research Laboratory, Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine Center, Michigan State University, East Lansing, MI 48824, USA.
Environ Health Perspect. 2003 Sep;111(12):1485-9. doi: 10.1289/ehp.6128.
Perfluorooctane sulfonate (PFOS) is a degradation product of sulfonyl-based fluorochemicals that are used extensively in industrial and household applications. Humans and wildlife are exposed to this class of compounds from several sources. Toxicity tests in rodents have raised concerns about potential developmental, reproductive, and systemic effects of PFOS. However, the effect of PFOS on the neuroendocrine system has not been investigated thus far. In this study, adult female rats were injected intraperitoneally with 0, 1, or 10 mg PFOS/kg body weight (BW) for 2 weeks. Food and water intake, BW, and estrous cycles were monitored daily. At the end of treatment, PFOS levels in tissues were measured by high-performance liquid chromatography (HPLC) interfaced with electrospray mass spectrometry. Changes in brain monoamines were measured by HPLC with electrochemical detection, and serum corticosterone and leptin were monitored using radioimmunoassay. Treatment with PFOS produced a dose-dependent accumulation of this chemical in various body tissues, including the brain. PFOS exposure decreased food intake and BW in a dose-dependent manner. Treatment with PFOS affected estrous cyclicity and increased serum corticosterone levels while decreasing serum leptin concentrations. PFOS treatment also increased norepinephrine concentrations in the paraventricular nucleus of the hypothalamus. These results indicate that exposure to PFOS can affect the neuroendocrine system in rats.
全氟辛烷磺酸（PFOS）是磺酰基类含氟化合物的降解产物，这类化合物广泛应用于工业和家庭领域。人类和野生动物通过多种途径接触此类化合物。对啮齿动物的毒性测试引发了人们对PFOS潜在的发育、生殖及全身影响的担忧。然而，迄今为止，PFOS对神经内分泌系统的影响尚未得到研究。在本研究中，成年雌性大鼠腹腔注射0、1或10毫克PFOS/千克体重，持续2周。每天监测食物和水摄入量、体重及发情周期。在治疗结束时，通过与电喷雾质谱联用的高效液相色谱法（HPLC）测量组织中的PFOS水平。采用具有电化学检测功能的HPLC测量脑单胺类物质的变化，并使用放射免疫分析法监测血清皮质酮和瘦素水平。PFOS处理导致该化学物质在包括脑在内的各种身体组织中呈剂量依赖性积累。PFOS暴露以剂量依赖性方式降低食物摄入量和体重。PFOS处理影响发情周期，提高血清皮质酮水平，同时降低血清瘦素浓度。PFOS处理还增加了下丘脑室旁核中的去甲肾上腺素浓度。这些结果表明，接触PFOS会影响大鼠的神经内分泌系统。
