Coleman Russell A, Bertolo Robert F, Moehn Soenke, Leslie Michael A, Ball Ronald O, Korver Doug R
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6G 2P5.
J Nutr. 2003 Sep;133(9):2826-9. doi: 10.1093/jn/133.9.2826.
The indicator amino acid oxidation (IAAO) method allows the determination of amino acid requirements under conditions of low growth rate as found in pre-laying broiler breeder pullets. Cobb 500 breeder pullets (20 wk old; 2290 +/- 280 g, n = 4) were adapted (6 d) to a pelleted, purified control diet containing all nutrients at >or=110% of NRC recommendations. After recovery from surgery for implantation of a jugular catheter, each bird was fed, in random order, test diets containing one of nine levels of lysine (0.48, 0.96, 1.92, 2.88, 3.84, 4.80, 7.68, 9.60 and 14.40 g/kg of diet). Indicator oxidation was determined during 4-h primed (74 kBq/kg body), constant infusions (44 kBq x h(-1). kg body(-1)) of L-[1-(14)C]phenylalanine. Using the breakpoint of a one-slope broken-line model, the lysine requirement was determined to be 4.88 +/- 0.96 g/kg of diet or 366 +/- 72 mg x hen(-1) x d(-1) with an upper 95% CI of 6.40 g/kg of diet or 480 mg x hen(-1) x d(-1). IAAO allows determination of individual bird amino acid requirements for specific ages and types of birds over short periods of time and enables more accurate broiler breeder pullet diet formulation.
指示性氨基酸氧化（IAAO）方法可用于测定低生长速率条件下的氨基酸需求量，如开产前肉种鸡小母鸡的情况。科宝500肉种鸡小母鸡（20周龄；体重2290±280克，n = 4）先适应（6天）一种颗粒状的纯化对照日粮，该日粮所含的所有营养素均≥NRC推荐量的110%。在颈静脉插管手术后恢复后，每只鸡按随机顺序喂食含有九种赖氨酸水平之一（0.48、0.96、1.92、2.88、3.84、4.80、7.68、9.60和14.40克/千克日粮）的试验日粮。在4小时内通过静脉注射L-[1-(14)C]苯丙氨酸（初始剂量74 kBq/千克体重，持续输注剂量44 kBq×h(-1)·kg体重(-1)）来测定指示性氧化。利用单斜率折线模型的转折点，确定赖氨酸需求量为4.88±0.96克/千克日粮或366±72毫克·只(-1)·天(-1)，95%置信上限为6.40克/千克日粮或480毫克·只(-1)·天(-1)。IAAO方法能够在短时间内测定特定年龄和类型鸡的个体氨基酸需求量，从而实现更精确的肉种鸡小母鸡日粮配方设计。
