Kozakiewicz Marcin, Godlewski Andrzej
Department of Maxillofacial Surgery, Institute of Surgery, Medical University of Łódź, Kopcińskiego 22, 90-153 Łódź, Poland.
Cell Mol Biol Lett. 2003;8(3):727-34.
To assess the effect of substances inducing mast cell degranulation (substance P and granuliberin R) on the mitotic indices of the gingiva stratified epithelium, basal cells from rats were studied in vivo. Seventy Lewis male rats were used in the study. The rats received injections of either 0.1 ml 0.9% NaCl (l0 rats), or substance P (10(-4), l0(-6), 10(-8) g/ml) (30 rats), or granuliberin R (10(-4), l0(-6), 10(-8) g/ml) (30 rats) into their mandibular gingiva in the vicinity of the right mental foramen. The mitotic index of keratinocytes was established after the kolchicine arrest (2 hours prior to material collection i.p. injection). The number of cells in metaphase was counted on 1000 consecutive basal layer cells after hematoxilin and eosin section staining. Mast cells were revealed using pinocyanol erythrosinate according to Bensley. Numerical density and morphometric features were analyzed. Substance P and granuliberin R injected into the gingiva affect the mast cells and the basal cell proliferation of the gingival epithelium. The diminished mitotic activity of basal layer cells was accompanied by degranulation and/or migration of mast cells under the basal membrane of the epithelium. After administration of high doses of granuloliberin R, mast cells were found in the deep connective tissue alligned towards the epithelium. A neuromediator from the trigeminal nerve (substance P) and substances from mast cells actively interfere in the proliferation of oral keratinocytes and the activity of connective tissue cells.
为评估诱导肥大细胞脱颗粒的物质（P物质和颗粒素R）对牙龈复层上皮有丝分裂指数的影响，对大鼠的基底细胞进行了体内研究。本研究使用了70只雄性Lewis大鼠。给大鼠在下颌右颏孔附近的牙龈注射0.1 ml 0.9%氯化钠（10只大鼠）、P物质（10(-4)、10(-6)、10(-8) g/ml）（30只大鼠）或颗粒素R（10(-4)、10(-6)、10(-8) g/ml）（30只大鼠）。在秋水仙碱阻滞（在腹腔注射采集材料前2小时）后确定角质形成细胞的有丝分裂指数。苏木精和伊红切片染色后，在1000个连续的基底层细胞中计数中期细胞的数量。根据本斯利法用玫瑰红派洛宁显示肥大细胞。分析了数值密度和形态计量学特征。注射到牙龈中的P物质和颗粒素R影响肥大细胞和牙龈上皮基底细胞的增殖。基底层细胞有丝分裂活性降低伴随着肥大细胞在基底膜下脱颗粒和/或迁移。给予高剂量颗粒素R后，在深层结缔组织中发现肥大细胞朝向上皮排列。三叉神经的神经介质（P物质）和肥大细胞的物质积极干扰口腔角质形成细胞的增殖和结缔组织细胞的活性。
