Song Miyeoun, Gutzeit Herwig O
Institute of Zoology, TU Dresden, Mommsenstrasse 13, D-01062 Dresden, Germany.
Dev Growth Differ. 2003 Aug;45(4):327-37. doi: 10.1046/j.1440-169x.2003.00701.x.
Organ cultures and primary cell cultures of medaka (Oryzias latipes) testis were compared with respect to cell viability and cell proliferation. The analysis by fluorescence microscopy and flow cytometry showed that in both cultures, the cells remained viable for at least 1 day and cell proliferation could be analyzed reliably by BrdU incorporation. The proliferating cells were mostly spermatogonia located at the periphery of the testis in tissue sections. Both culture systems were used to study the effect of 17-alpha-ethynylestradiol on cell proliferation. The results obtained with organ and primary cultures were consistent: low concentrations (0.01 and 1 nm) of synthetic estrogen stimulated cell proliferation slightly, while a higher concentration (100 nm) had an inhibitory effect. Both culture methods are suitable for the analysis of substances that might interfere with germ cell proliferation or other functions in spermatogenesis.
对青鳉（Oryzias latipes）睾丸的器官培养和原代细胞培养进行了细胞活力和细胞增殖方面的比较。荧光显微镜和流式细胞术分析表明，在两种培养物中，细胞至少可存活1天，并且通过掺入BrdU能够可靠地分析细胞增殖情况。在组织切片中，增殖细胞大多是位于睾丸周边的精原细胞。两种培养系统均用于研究17-α-乙炔基雌二醇对细胞增殖的影响。器官培养和原代培养获得的结果一致：低浓度（0.01和1纳米）的合成雌激素对细胞增殖有轻微刺激作用，而高浓度（100纳米）则具有抑制作用。两种培养方法都适用于分析可能干扰生殖细胞增殖或精子发生中其他功能的物质。
