Song Hyuk, Park Hyun-Jung, Lee Won-Young, Lee Kyung Hoon
Department of Stem Cell and Regenerative Technology, KIT, Konkuk University, Seoul 05029, Republic of Korea.
Department of Animal Biotechnology, College of Life Science and Natural Resources, Sangji University, Wonju-si 26339, Republic of Korea.
Stem Cells Int. 2022 May 31;2022:4755514. doi: 10.1155/2022/4755514. eCollection 2022.
Spermatogonial stem cells (SSCs) are the germline stem cells that are essential for the maintenance of spermatogenesis in the testis. However, it has not been sufficiently understood in amphibians, reptiles, and fish because numerous studies have been focused mainly on mammals. The aim of this review is to discuss scientific ways to elucidate SSC models of nonmammals in the context of the evolution of testicular organization since rodent SSC models. To further understand the SSC models in nonmammals, we point out common markers of an SSC pool (undifferentiated spermatogonia) in various types of testes where the kinetics of the SSC pool appears. This review includes the knowledge of (1) common molecular markers of vertebrate type A spermatogonia including putative SSC markers, (2) localization of the markers on the spermatogonia that have been reported in previous studies, (3) highlighting the most common markers in vertebrates, and (4) suggesting ways of finding SSC models in nonmammals.
精原干细胞（SSCs）是种系干细胞，对于维持睾丸中的精子发生至关重要。然而，在两栖动物、爬行动物和鱼类中，人们对此尚未有充分的了解，因为众多研究主要集中在哺乳动物身上。本综述的目的是讨论自啮齿动物精原干细胞模型以来，在睾丸组织进化的背景下阐明非哺乳动物精原干细胞模型的科学方法。为了进一步了解非哺乳动物的精原干细胞模型，我们指出了出现精原干细胞库动力学的各种类型睾丸中精原干细胞库（未分化精原细胞）的共同标志物。本综述包括以下方面的知识：（1）脊椎动物A型精原细胞的共同分子标志物，包括假定的精原干细胞标志物；（2）先前研究中报道的标志物在精原细胞上的定位；（3）突出脊椎动物中最常见的标志物；（4）提出在非哺乳动物中寻找精原干细胞模型的方法。
