Burchardt E R, Hein R, Bosserhoff A K
fibroSys, Wuppertal, and Institute of Clinical Pharmacology, University of Witten-Herdecke, Witten-Herdecke, Germany.
Clin Exp Dermatol. 2003 Sep;28(5):515-20. doi: 10.1046/j.1365-2230.2003.01326.x.
In this study, hyaluronan, laminin-1, tenascin-C and type VI collagen were measured in the sera of patients with stage I/II and stage IV melanoma. A significant increase in the serum levels of all four extracellular matrix proteins was found in patients with stage IV melanoma compared to healthy donors. Type VI collagen and hyaluronan serum levels were also significantly increased in stage I/II melanoma. Increased expression of the four matrix proteins was also demonstrated in melanoma cell lines using reverse transcriptase- polmerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). We suggest that tenascin-C, hyaluronan, laminin-1 and type VI collagen are involved in melanoma development and extracellular matrix remodelling during melanoma progression. This finding will be of interest in the development of serum markers for progression of malignant melanoma.
在本研究中，对I/II期和IV期黑色素瘤患者血清中的透明质酸、层粘连蛋白-1、肌腱蛋白-C和VI型胶原进行了检测。与健康供体相比，IV期黑色素瘤患者血清中所有四种细胞外基质蛋白水平均显著升高。I/II期黑色素瘤患者血清中VI型胶原和透明质酸水平也显著升高。使用逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）在黑色素瘤细胞系中也证实了这四种基质蛋白的表达增加。我们认为，肌腱蛋白-C、透明质酸、层粘连蛋白-1和VI型胶原参与了黑色素瘤的发展以及黑色素瘤进展过程中的细胞外基质重塑。这一发现对于恶性黑色素瘤进展血清标志物的开发具有重要意义。
