Erlandsen S, Chen Y, Frethem C, Detry J, Wells C
Department of Genetics, Cell Biology and Development, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
J Microsc. 2003 Sep;211(Pt 3):212-8. doi: 10.1046/j.1365-2818.2003.01218.x.
High-resolution backscatter electron (BSE) imaging of colloidal gold can be accomplished at low voltage using in-lens or below-the-lens FESEMs equipped with either Autrata-modified yttrium aluminium garnet (YAG) scintillators doped with cerium, or with BSE to secondary electron (SE) conversion plates. The threshold for BSE detection of colloidal gold was 1.8 keV for the YAG detector, and the BSE/SE conversion was sensitive down to 1 keV. Gold particles (6, 12 and 18 nm) have an atomic number of 79 and were clearly distinguished at 500,000x by materials contrast and easily discriminated from cell surfaces coated with platinum with an atomic number of 78. BSE imaging was relatively insensitive to charging, and build up of carbon contamination on the specimen was transparent to the higher energy BSE.
使用配备Autrata改性的掺铈钇铝石榴石（YAG）闪烁体或背散射电子（BSE）到二次电子（SE）转换板的透镜内或透镜下场发射扫描电子显微镜（FESEM），可以在低电压下完成胶体金的高分辨率背散射电子（BSE）成像。对于YAG探测器，检测胶体金的BSE阈值为1.8 keV，BSE/SE转换在低至1 keV时仍很灵敏。金颗粒（6、12和18 nm）的原子序数为79，通过材料对比度在500,000倍放大倍数下清晰可辨，并且很容易与原子序数为78的铂涂层细胞表面区分开来。BSE成像对充电相对不敏感，并且样品上碳污染的积累对较高能量的BSE是透明的。
