Baldwin M K, Berry P H, Esdaile D J, Linnett S L, Martin J G, Peristianis G C, Priston R A, Simpson B J, Smith J D
Shell Research Ltd., Sittingbourne Research Centre, Kent, United Kingdom.
Toxicol Pathol. 1992;20(3 Pt 1):426-35. doi: 10.1177/019262339202000312.
This investigation compared the effects of feeding rats diets containing food grade white oil processed by either conventional oleum treatment or the more modern method of catalytic hydrogenation. In two separate experiments, male or female Fischer-344 rats were given free access for 90 days to diets containing 0, 10, 100, 500, 5,000, 10,000, or 20,000 ppm of either oleum-treated white oil (OTWO) or hydrotreated white oil (HTWO). There were no mortalities and no adverse clinical signs associated with feeding either white oil. Treatment-related effects evidenced by hematological, clinical chemical, and pathological changes were generally dose-related and more marked in female than in male rats, and the OTWO caused a greater pathological response than the HTWO. Tissue residues of saturated hydrocarbons were up to 5.2 times higher in female rats than in males. Rats fed 5,000 ppm or more of either white oil showed dose-related alterations in several hematological and clinical chemistry variates associated mainly with hepatic damage or functional alteration. At necropsy, mesenteric lymph nodes were enlarged, and increases in weight of liver, kidney, and spleen were significant. Microscopic changes were characterized by multifocal lipogranulomata in mesenteric lymph node and liver. No changes were observed in rats fed OTWO or HTWO for 90 days at dietary concentrations of 10 or 100 ppm, equivalent to a minimum intake of 0.65 and 6.4 mg/kg/day, respectively. Differences in degree of pathological response associated with each oil may have been due to their differences in specification rather than processing method.
本研究比较了给大鼠喂食含有通过传统发烟硫酸处理或更现代的催化氢化方法加工的食品级白油的日粮所产生的影响。在两项独立实验中，雄性或雌性Fischer-344大鼠可自由摄取含0、10、100、500、5000、10000或20000 ppm发烟硫酸处理白油（OTWO）或加氢处理白油（HTWO）的日粮，为期90天。喂食两种白油均未导致死亡，也未出现不良临床症状。血液学、临床化学和病理学变化所证明的与处理相关的影响通常与剂量相关，且在雌性大鼠中比在雄性大鼠中更明显，并且OTWO引起的病理反应比HTWO更大。雌性大鼠体内饱和烃的组织残留量比雄性大鼠高5.2倍。喂食5000 ppm或更高剂量任一种白油的大鼠在几个主要与肝损伤或功能改变相关的血液学和临床化学变量上出现了与剂量相关的变化。尸检时，肠系膜淋巴结肿大，肝脏、肾脏和脾脏重量显著增加。微观变化的特征是肠系膜淋巴结和肝脏出现多灶性脂肉芽肿。在日粮浓度为10或100 ppm（分别相当于最低摄入量0.65和6.4 mg/kg/天）的情况下，喂食OTWO或HTWO 90天的大鼠未观察到变化。与每种油相关的病理反应程度的差异可能是由于它们规格上的差异而非加工方法的差异。
