Lee Ji Yeoun, Kang Won Hyoung
Department of Dermatology, Ajou University School of Medicine, Suwon, South Korea.
Pigment Cell Res. 2003 Oct;16(5):504-8. doi: 10.1034/j.1600-0749.2003.00081.x.
Cyclosporin A (CsA) is a widely used immunosuppressant. Reports on the effect of CsA on hyperpigmentation in patients appear inconsistent, and the effect of CsA on skin pigment cells (melanocytes) in vitro is unknown. We examined the effect of CsA on human melanocyte proliferation and melanogenesis in vitro. Melanocyte proliferation was dose-dependently inhibited by 0.1-10 microM CsA, with no effect on cell viability. Melanocytes incubated with 10 microM CsA for 6 days showed decreased pigmentation and tyrosinase activity. Western blot analysis using an anti-tyrosinase antibody revealed that CsA (0.1-10 microM) decreased tyrosinase protein levels in a dose-dependent manner. Northern blot analysis showed similar effects on tyrosinase mRNA levels. These effects of CsA on melanogenesis in vitro are not consistent with suggestions that systemic CsA therapy causes patient skin hyperpigmentation.
环孢素A（CsA）是一种广泛使用的免疫抑制剂。关于CsA对患者色素沉着影响的报道似乎并不一致，且CsA对体外皮肤色素细胞（黑素细胞）的影响尚不清楚。我们在体外研究了CsA对人黑素细胞增殖和黑素生成的影响。0.1 - 10微摩尔的CsA对黑素细胞增殖有剂量依赖性抑制作用，对细胞活力无影响。用10微摩尔CsA孵育6天的黑素细胞色素沉着和酪氨酸酶活性降低。使用抗酪氨酸酶抗体的蛋白质印迹分析表明，CsA（0.1 - 10微摩尔）以剂量依赖性方式降低酪氨酸酶蛋白水平。Northern印迹分析显示对酪氨酸酶mRNA水平有类似影响。CsA对体外黑素生成的这些影响与全身性CsA治疗导致患者皮肤色素沉着的观点不一致。
