β-乳球蛋白抑制培养的人黑素细胞中的黑色素生成。

Beta-lactoglobulin suppresses melanogenesis in cultured human melanocytes.

作者信息

Nakajima M, Shinoda I, Mikogami T, Iwamoto H, Hashimoto S, Miyauchi H, Fukuwatari Y, Hayasawa H

机构信息

Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan.

出版信息

Pigment Cell Res. 1997 Dec;10(6):410-3. doi: 10.1111/j.1600-0749.1997.tb00700.x.

DOI:10.1111/j.1600-0749.1997.tb00700.x

PMID:9428009

Abstract

The effects of whey proteins from bovine milk on melanogenesis in cultured human melanocytes were examined. Among the major protein components of milk whey including beta-lactoglobulin (BLG), alpha-lactalbumin, serum albumin, and IgG, only BLG exhibited the depigmenting effect at a concentration of 1 mg/ml. Also, BLG suppressed the activity of tyrosinase in these cells. Retinol, to which BLG is known to bind, slightly increased the pigmentation of the cells at concentrations in the range of 1-100 nM, and retinoic acid, a metabolite of retinol, exhibited a strong pigmentation-promoting effect within the same concentration range. Treatment of the cells with 1 mg/ml BLG completely abrogated the pigmentation induced by these A vitamins. These results demonstrate a novel biological activity of BLG and suggest that this activity is dependent on its ability to bind retinol.

摘要

研究了牛乳清蛋白对培养的人黑素细胞黑素生成的影响。在乳清的主要蛋白质成分中，包括β-乳球蛋白（BLG）、α-乳白蛋白、血清白蛋白和IgG，只有BLG在浓度为1mg/ml时表现出脱色作用。此外，BLG抑制了这些细胞中酪氨酸酶的活性。已知BLG与之结合的视黄醇，在1-100nM的浓度范围内会使细胞的色素沉着略有增加，而视黄醇的代谢产物视黄酸在相同浓度范围内表现出强烈的促色素沉着作用。用1mg/ml BLG处理细胞完全消除了这些维生素A诱导的色素沉着。这些结果证明了BLG的一种新的生物学活性，并表明这种活性取决于其结合视黄醇的能力。