Iwai-Liao Y, Higashi Y, Ishikawa M, Hori H
Dept. Oral Anatomy, Electron Microscope Facility, Osaka Dental University, Japan.
Scanning Microsc. 1992 Dec;6(4):1089-95; discussion 1095-6.
Intracellular structures of the odontoblasts were studied by scanning electron microscopy (SEM) using a modified AODO (aldehyde prefixed-osmium-DMSO-osmium) method. Well-developed flattened and layered rER (rough endoplasmic reticulum), paved with its associated ribosomes on its outer surface, were clearly observed in the odontoblast. Branched tubular mitochondria with nodules and swollen endings, interposing between and passing through the fenestrated layered rER, were demonstrated in the functional cells. Oblique and cross-sections of both the rER system and tubular mitochondria showed orthodox configurations similar to those usually described in transmission electron microscopy (TEM) studies. Many finger-like projections constructing the cristae directing towards the inner mitochondrial chamber were observed, and external chamber extending into the tubular cristae was also demonstrated.
采用改良的AODO（醛预固定-锇-DMSO-锇）方法，通过扫描电子显微镜（SEM）对成牙本质细胞的细胞内结构进行了研究。在成牙本质细胞中清晰观察到发育良好的扁平且分层的粗面内质网（rER），其外表面排列着相关的核糖体。在功能细胞中发现了带有结节和肿胀末端的分支管状线粒体，它们介于有孔分层的rER之间并穿过rER。rER系统和管状线粒体的斜切面和横截面显示出与通常在透射电子显微镜（TEM）研究中描述的正统结构相似。观察到许多构成嵴的指状突起指向线粒体内腔，并且还显示出延伸到管状嵴中的外腔。
