Tanaka K, Mitsushima A, Fukudome H, Kashima Y
J Submicrosc Cytol. 1986 Jan;18(1):1-9.
In spite of many light and transmission electron microscopic studies of the Golgi complex, many important points remain unsettled. Recently, we have devised a method of observing the three-dimensional configurations of intracellular structures by scanning electron microscopy. We have used this method to study the three-dimensional structure of the Golgi complex in several tissues of the rat, paying special attention to three problems: the overall configuration; the spatial construction of each stack, and the relationship to neighbouring organelles. The interconnected stacks which form the Golgi complex are arranged in a manner characteristic of the particular cell type. In rat extraorbital lacrimal gland the stacks form an irregular network spread throughout the cell, while in anterior pituitary cells they form a hollow sphere. Continuities were seen between cisternae within a stack, and in one case a stack actually consisted of one helically wound cisterna. We also observed slender tubules connecting Golgi stacks to neighbouring rough endoplasmic reticulum. Vesicles, presumably transport vesicles, were frequently observed on the surface of the cisternae, most commonly at the cisternal rims. However the continuities among stacks and connections with cisternae and with the rough endoplasmic reticulum demonstrated here offer alternative routes for movement of substances through the cellular endomembrane system.
尽管对高尔基体进行了大量的光学显微镜和透射电子显微镜研究，但许多重要问题仍未解决。最近，我们设计了一种通过扫描电子显微镜观察细胞内结构三维构型的方法。我们已使用该方法研究大鼠几种组织中高尔基体的三维结构，特别关注三个问题：整体构型；每个堆叠的空间结构，以及与相邻细胞器的关系。构成高尔基体的相互连接的堆叠以特定细胞类型特有的方式排列。在大鼠眶外泪腺中，堆叠形成遍布细胞的不规则网络，而在垂体前叶细胞中，它们形成空心球体。在一个堆叠内的池之间观察到连续性，在一个案例中，一个堆叠实际上由一个螺旋缠绕的池组成。我们还观察到细长的小管将高尔基体堆叠连接到相邻的粗面内质网。在池的表面经常观察到囊泡，大概是运输囊泡，最常见于池的边缘。然而，这里展示的堆叠之间的连续性以及与池和粗面内质网的连接为物质通过细胞内膜系统的移动提供了替代途径。
