Kim Hyongbum, Kim Hyun Woo, Suh Hwal
Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-ku, Seoul 120-752, South Korea.
Biomaterials. 2003 Nov;24(25):4671-9. doi: 10.1016/s0142-9612(03)00358-2.
The purpose of this research was to develop porous poly(D,L-lactide-co-glycolide) (PLGA) scaffolds from which ascorbate-2-phosphate (AsAP) and dexamethasone (Dex) are continuously released for a month for osteogenesis of mesenchymal stem cells for bone tissue engineering. Porous PLGA matrices containing AsAP and Dex were prepared by solvent casting/particulate leaching method. In vitro release and water uptake studies were performed in Dulbecco's phosphate buffered saline at 37 degrees C and 15 rpm. Drug loading and release rates were determined by high performance liquid chromatography. Release studies of Dex and AsAP showed that, after an initial burst release lasting 4 and 9 days, respectively, release rates followed zero order kinetics with high correlation coefficients at least until 35 days. Incorporation of AsAP into the scaffolds increased the release rates of Dex and AsAP, and the scaffold water uptake. When mesenchymal stem cells (MSCs) were cultured in the AsAP and Dex containing scaffolds in vitro, the amount of mineralization was significantly higher than in control scaffolds. In conclusion, AsAP and Dex were incorporated into porous PLGA scaffolds and continuously released over a month and osteogenesis of MSCs was increased by culture in these scaffolds.
本研究的目的是开发多孔聚（D，L-丙交酯-共-乙交酯）（PLGA）支架，从该支架中持续释放抗坏血酸-2-磷酸酯（AsAP）和地塞米松（Dex）一个月，用于间充质干细胞的成骨作用，以用于骨组织工程。通过溶剂浇铸/颗粒沥滤法制备了含有AsAP和Dex的多孔PLGA基质。在37℃和15转/分钟的条件下，于杜尔贝科磷酸盐缓冲盐水中进行体外释放和吸水率研究。通过高效液相色谱法测定药物负载量和释放速率。Dex和AsAP的释放研究表明，在分别持续4天和9天的初始突释之后，释放速率遵循零级动力学，至少直到35天具有高相关系数。将AsAP掺入支架中增加了Dex和AsAP的释放速率以及支架的吸水率。当在体外将间充质干细胞（MSCs）培养在含有AsAP和Dex的支架中时，矿化量显著高于对照支架。总之，将AsAP和Dex掺入多孔PLGA支架中并持续释放一个月，并且通过在这些支架中培养增加了MSCs的成骨作用。
