Gieske T H, Baugh L E
Marion Merrell Dow Research Institute, Cincinnati, OH 45215.
Agents Actions. 1992 Nov;37(3-4):198-200. doi: 10.1007/BF02028108.
Acute airway hyperresponsiveness can be induced after exposure to aerosolized ovalbumin in sensitized guinea pigs. The purpose of the present studies was to determine if "pro-inflammatory agents" would potentiate and prolong antigen-induced pulmonary hyperresponsiveness to histamine in guinea pigs. Guinea pigs were sensitized to aerosolized ovalbumin by exposing them to a 3 min aerosol, generated ultrasonically from a 10% ovalbumin solution on day 0 and day 7. On day 13 the guinea pigs were exposed to a 3 min aerosol of deionized water or a pro-inflammatory agent (1 microgram/ml PAF, 1 mg/ml LPS, or 4% B. pertussis vaccine). Twenty-four hours later, on day 14, the conscious guinea pigs were challenged with a 3 min aerosolized ovalbumin exposure (under isoproterenol cover) and the individual guinea pig responsiveness to aerosolized histamine was determined 2 and 24 h later in an anesthetized modified Konzett-Rossler preparation. Under these experimental conditions, ovalbumin challenge to sensitized guinea pigs produced only an acute hyperresponsiveness (about a 3-10-fold shift) to aerosolized histamine, which lasted less than 24 h. The pro-inflammatory agents neither potentiated nor prolonged the duration of the hyperresponsiveness.
在致敏豚鼠中，暴露于雾化卵清蛋白后可诱发急性气道高反应性。本研究的目的是确定“促炎剂”是否会增强并延长豚鼠抗原诱导的对组胺的肺高反应性。通过在第0天和第7天将豚鼠暴露于由10%卵清蛋白溶液超声产生的3分钟气雾剂中，使其对雾化卵清蛋白致敏。在第13天，将豚鼠暴露于3分钟的去离子水气雾剂或促炎剂（1微克/毫升血小板活化因子、1毫克/毫升脂多糖或4%百日咳疫苗）中。24小时后，即第14天，对清醒的豚鼠进行3分钟雾化卵清蛋白暴露挑战（在异丙肾上腺素保护下），并在2小时和24小时后，在麻醉的改良Konzett-Rossler装置中测定个体豚鼠对雾化组胺的反应性。在这些实验条件下，对致敏豚鼠进行卵清蛋白激发仅产生对雾化组胺的急性高反应性（约3至10倍的变化），持续时间不到24小时。促炎剂既没有增强高反应性，也没有延长其持续时间。
