Stefan Raluca-Ioana, Bokretsion Rahel Girmai
Department of Chemistry, University of Pretoria, Pretoria, South Africa.
J Immunoassay Immunochem. 2003;24(3):319-24. doi: 10.1081/IAS-120022941.
An amperometric immunosensor, based on diamond paste (diamond powder and paraffin oil), has been constructed for the assay of azidothymidine (AZT). The diamond paste is impregnated with anti-AZT. The immunosensor can be used reliably for the assay of azidothymidine in its pharmaceutical formulations. The potential used for azidothymidine assay was + 240 mV vs. Ag/AgCl electrode. The surface of the immunosensor can be regenerated by simply polishing, thereby obtaining fresh immunocomposite ready to be used in a new assay. The new amperometric immunosensor, based on diamond paste, gives reliable results for the assay of AZT as raw material and from its pharmaceutical formulation.
一种基于金刚石糊剂（金刚石粉末和石蜡油）的安培免疫传感器已被构建用于测定齐多夫定（AZT）。金刚石糊剂中浸渍了抗AZT。该免疫传感器可可靠地用于其药物制剂中齐多夫定的测定。测定齐多夫定时使用的电位相对于Ag/AgCl电极是 +240 mV。免疫传感器的表面可通过简单抛光进行再生，从而获得可用于新测定的新鲜免疫复合物。这种基于金刚石糊剂的新型安培免疫传感器，对于作为原料及其药物制剂中的AZT测定能给出可靠结果。
