Li Chonghua, Geng Qiuming, Nicolau David P, Nightingale Charles H
Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT 06102, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Sep 5;794(2):227-36. doi: 10.1016/s1570-0232(03)00456-2.
A gradient elution HPLC method with a wavelength switch technique was developed to simultaneously analyze the beta-lactam ticarcillin and the beta-lactamase inhibitor clavulanate in rabbit serum and tissue cage fluid (TCF). A C18 reversed-phase column with a programmable UV detector changing the wavelength from 218 to 254 nm at 9 min was used for chromatographic separation. The mobile phase consisted of acetonitrile, phosphate buffer and tetrabutylammonium hydrogen sulfate by following a gradient elution program at a flow-rate of 1 ml/min. Sample processing was carried out with liquid-liquid extraction. Good linearity, recoveries, precision and accuracy were obtained. The ranges of the standard curves were 1-100 microg/ml for ticarcillin, and 0.2-20 microg/ml for clavulanate. This assay has been successfully applied to analyze ticarcillin and clavulanate in rabbit serum and tissue cage fluid samples from a pharmacokinetic study.
建立了一种采用波长切换技术的梯度洗脱高效液相色谱法，用于同时分析兔血清和组织笼液（TCF）中的β-内酰胺类替卡西林和β-内酰胺酶抑制剂克拉维酸。使用C18反相柱和可编程紫外检测器，该检测器在9分钟时将波长从218 nm切换至254 nm进行色谱分离。流动相由乙腈、磷酸盐缓冲液和硫酸氢四丁铵组成，按照梯度洗脱程序，流速为1 ml/min。采用液-液萃取进行样品处理。获得了良好的线性、回收率、精密度和准确度。替卡西林标准曲线范围为1-100 μg/ml，克拉维酸标准曲线范围为0.2-20 μg/ml。该测定法已成功应用于药代动力学研究中兔血清和组织笼液样品中替卡西林和克拉维酸的分析。
