通过液液萃取和反相高效液相色谱法对小鼠血清和支气管肺泡灌洗液中的阿莫西林进行灵敏测定。
A sensitive assay of amoxicillin in mouse serum and broncho-alveolar lavage fluid by liquid-liquid extraction and reversed-phase HPLC.
作者信息
Du Xiaoli, Li Chonghua, Sun Heather K, Nightingale Charles H, Nicolau David P
机构信息
Center for Anti-Infective Research and Development, Hartford Hospital, 80 Seymour Street, Hartford, CT 06102, USA.
出版信息
J Pharm Biomed Anal. 2005 Sep 15;39(3-4):648-52. doi: 10.1016/j.jpba.2005.04.021.
A sensitive and simple high-performance liquid chromatography (HPLC) method was developed and validated for the analysis of amoxicillin in mouse serum and broncho-alveolar lavage (BAL) fluid. One hundred microlitres of sample were needed for the assay. Sample processing was carried out with liquid-liquid extraction. Cefadroxil was used as an internal standard. The chromatographic separation was achieved on a C18 reversed-phase column with a mobile phase consisting of phosphate buffer, 1-octanesulphonic acid sodium salt and acetonitrile. The detection was conducted at 210 nm. The ranges of the standard curves were 0.2-20 and 0.05-5 microg/ml for serum and BAL samples, respectively. The recoveries of amoxicillin from serum and normal saline were 87 and 88%, respectively. The coefficients of variation were 1.78-6.13% for intra-day and 0.82-6.42% for inter-day analyses. The accuracy was within 100+/-6%. This method was successfully applied to analyze amoxicillin in mouse serum and BAL samples from a pharmacokinetic study.
建立了一种灵敏且简便的高效液相色谱（HPLC）法，并对其进行了验证，用于分析小鼠血清和支气管肺泡灌洗（BAL）液中的阿莫西林。该测定需要100微升样品。采用液液萃取进行样品处理。头孢羟氨苄用作内标。在C18反相柱上进行色谱分离，流动相由磷酸盐缓冲液、1-辛烷磺酸钠盐和乙腈组成。检测波长为210nm。血清和BAL样品的标准曲线范围分别为0.2 - 20和0.05 - 5微克/毫升。阿莫西林在血清和生理盐水中的回收率分别为87%和88%。日内分析的变异系数为1.78 - 6.13%，日间分析的变异系数为0.82 - 6.42%。准确度在100±6%以内。该方法成功应用于药代动力学研究中对小鼠血清和BAL样品中阿莫西林的分析。
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