Shultz T D, Chew B P, Seaman W R
Department of Food Science and Human Nutrition, Washington State University, Pullman 99164-6376.
Anticancer Res. 1992 Nov-Dec;12(6B):2143-5.
Consumption of dietary fat has been linked to the high incidence of certain cancers. However, recent research has stimulated interest in conjugated linoleic acid (CLA), a newly recognized anticarcinogenic fatty acid. Human MCF-7 breast cancer cells were incubated for 12 d in culture medium supplemented with various concentrations (1.78-7.14 x 10(-5) M) of linoleic acid (LA) or CLA. Linoleic acid initially stimulated MCF-7 cell growth with an optimal effect at concentrations of 3.57-7.14 x 10(-5) M, but was inhibitory at similar concentrations after 8 and 12 d of incubation. In contrast, CLA was inhibitory to cancer cell growth at all concentrations and times tested. Cell growth inhibition by CLA was dose- and time-dependent. Growth retardation at the prescribed LA and CLA concentrations ranged, respectively, from 4 to 33% and 54 to 100% following 8 to 12 d of treatment. At similar LA and CLA concentrations, cytostatic and cytotoxic effects of CLA were more pronounced (8-81%) than LA. These in vitro results suggest that CLA is cytotoxic to MCF-7 cells.
膳食脂肪的摄入与某些癌症的高发病率有关。然而，最近的研究激发了人们对共轭亚油酸（CLA）的兴趣，CLA是一种新发现的具有抗癌作用的脂肪酸。将人MCF-7乳腺癌细胞在补充了不同浓度（1.78 - 7.14×10⁻⁵ M）亚油酸（LA）或CLA的培养基中培养12天。亚油酸最初刺激MCF-7细胞生长，在浓度为3.57 - 7.14×10⁻⁵ M时效果最佳，但在培养8天和12天后，在相似浓度下具有抑制作用。相比之下，CLA在所有测试浓度和时间下均抑制癌细胞生长。CLA对细胞生长的抑制作用具有剂量和时间依赖性。在8至12天的处理后，规定的LA和CLA浓度下的生长迟缓分别为4%至33%和54%至100%。在相似的LA和CLA浓度下，CLA的细胞生长抑制和细胞毒性作用（8% - 81%）比LA更明显。这些体外实验结果表明CLA对MCF-7细胞具有细胞毒性。
