顺-9，反-11-共轭亚油酸诱导人乳腺癌（MCF-7）细胞凋亡的作用

[Effect of apoptosis in human mammary cancer (MCF-7) cells induced by cis9, t11-conjugated linoleic acid].

作者信息

Liu Jiaren, Chen Bingqing, Yang Yanmei, Wang Xuanlin

机构信息

Public Health College, Harbin Medical University, Harbin 150001, China.

出版信息

Wei Sheng Yan Jiu. 2004 Jan;33(1):58-62.

PMID:15098480

Abstract

OBJECTIVE

To determine the effect of cell apoptosis induced by c9, t11-conjugated linoleic acid was investigated in human mammary cancer cells (MCF-7), which were treated with various c9, t11-CLA concentrations (25, 50, 100 and 200 mumol/L), with a negative control.

METHODS

In this study, the methods of the curve of cell growth, cell apoptosis detecting in fluorescent and electronic microscope and flow cytometry, and expression of p53 protein were employed.

RESULTS

The cell growth of MCF-7 cells was inhibited by c9, t11-CLA. Eight day after treatment with various concentrations of c9, t11-CLA, as mentioned above, the inhibition rates were -6.0%, 45.2%, 99.0% and 99.4%, respectively. Apoptotic cells of morphology from MCF-7 cells treated by different concentrations of c9, t11-CLA were observed by fluorescent and electronic microscope and the frequency of apoptosis in MCF-7 cells by flow cytometry showed increasingly with concentrations of c9, t11-CLA increased. While the expression of p53 protein in MCF-7 cells was decreased with the doses of c9, t11-CLA increased.

CONCLUSION

c9, t11-CLA can induce the effect of apoptosis in MCF-7 cells and its may be one of mechanisms for the inhibitory effect of c9, t11-CLA in human cancer cells.

摘要

目的

研究c9，t11-共轭亚油酸（c9，t11-CLA）对人乳腺癌细胞（MCF-7）凋亡的影响，将其用不同浓度（25、50、100和200μmol/L）的c9，t11-CLA处理，并设阴性对照。

方法

本研究采用细胞生长曲线、荧光和电子显微镜下细胞凋亡检测、流式细胞术以及p53蛋白表达检测等方法。

结果

c9，t11-CLA抑制MCF-7细胞的生长。用上述不同浓度的c9，t11-CLA处理8天后，抑制率分别为-6.0%、45.2%、99.0%和99.4%。通过荧光和电子显微镜观察不同浓度c9，t11-CLA处理的MCF-7细胞的凋亡形态，流式细胞术检测显示MCF-7细胞的凋亡频率随c9，t11-CLA浓度增加而升高。而MCF-7细胞中p53蛋白的表达随c9，t11-CLA剂量增加而降低。