Edwards Christine, Liu Jianbo, Smith Thomas J, Brooke Daniel, Hunter David J, Organ Andrew, Coffey Patrick
Biotage, Inc., A Dyax Corp Company, P.O. Box 8006, Charlottesville, VA 22906, USA.
Rapid Commun Mass Spectrom. 2003;17(18):2027-33. doi: 10.1002/rcm.1146.
High-throughput chemistry (HTC) is now an integral part of the lead discovery process in many pharmaceutical and related chemical companies. As this process becomes refined or improved, with the integration of systems with enhanced capabilities, and the requirement for quality compounds of high purity increases, purification is often considered a bottleneck. Although a wide range of purification techniques is available, high-performance liquid chromatography (HPLC) is usually the preferred method of purification to produce high-purity compounds. Parallel preparative HPLC with robust UV-guided fraction collection has been shown to reduce the bottleneck and complement the parallel synthesis systems. However, despite the success of this technique, post-purification analysis of fractions to identify the target compound adds an additional level of complexity. This paper describes the interfacing of the Biotage Parallex with the MUX interface on a single quadrupole mass spectrometer, thus combining robust UV-guided fractionation with on-line MS characterization.
高通量化学（HTC）如今在许多制药及相关化工企业的先导化合物发现过程中已不可或缺。随着这一过程不断完善或改进，随着具备更强功能的系统整合以及对高纯度优质化合物需求的增加，纯化常常被视为一个瓶颈。尽管有多种纯化技术可供选择，但高效液相色谱法（HPLC）通常是生产高纯度化合物时首选的纯化方法。具有强大紫外引导馏分收集功能的平行制备型HPLC已被证明可减少瓶颈并补充平行合成系统。然而，尽管该技术取得了成功，但对馏分进行纯化后分析以鉴定目标化合物增加了额外的复杂程度。本文描述了Biotage Parallex与单四极杆质谱仪上的MUX接口的连接，从而将强大的紫外引导分馏与在线质谱表征相结合。
