Cloning, characterization and partial sequence analysis of the 5'-flanking region of bovine alpha-sl casein gene.

We have isolated a clone from a EMBL3 constructed bovine genomic library using a PCR-generated DNA fragment as a probe covering-660bp to + 158 bp of boving alpha-sl casein gene. The results obtained from hybridization assay, restriction endonucleases map and partial DNA sequence analysis indicated that the cloned fragment spanned 5.7kb of the first part of the gene and 8.0 kb of its upstream flanking region. Some divergent restriction endonuclease recognition sites and a few mutations and deletions were found in exons, introns and 5'-flanking region of the gene we cloned when compared with the PCR-generated fragment and those which had been published [6,10]. All the deletions occurred at repeat sequences.