Murakami Satoshi, Koike Yoshihiko, Matsuzaka Kenichi, Ohata Hitoshi, Uchiyama Takeshi, Inoue Takashi
Department of Clinical Pathophysiology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan.
Bull Tokyo Dent Coll. 2003 May;44(2):61-6. doi: 10.2209/tdcpublication.44.61.
The purpose of this study was to investigate a case of calcifying odontogenic cyst (COC) in which numerous calcifications were observed not only in the lining epithelium, but also in the cyst wall, using cytokeratins 13 (CK13), 19 (CK19), and core binding factor a-1 (cbfa-1) as primary antibodies. Cells of Malassez's epithelial rest were stained as controls. Cells of the epithelial nests in the cyst wall were reactive for CK13, but their CK19 staining was similar to that observed in the lining epithelial cells. Calcifying nodules were reactive only for CK13. Cells of Malassez's epithelial rest were reactive for CK19 but not for CK13. Cbfa-1 positive reactivity was observed only in nuclei of spindle cells in the periodontal ligament. CK13 was positive superficial to the prickle cells. CK19 was positive in the basal cells of the oral mucosa. In the lining epithelium of the cyst, the expressions of CK13 and CK19 were similar to their immunoreactions in the oral mucosa. These results suggest that the odontogenic epithelium differentiated into squamous epithelial cells, which began as ghost cells in the COC, and that this process depended on the dystrophic calcification of differentiated odontogenic epithelial cells, not of osteogenic cells.
本研究的目的是利用细胞角蛋白13（CK13）、19（CK19）和核心结合因子α-1（cbfa-1）作为一抗，研究一例牙源性钙化囊肿（COC），其中不仅在衬里上皮中观察到大量钙化，在囊壁中也观察到大量钙化。将马拉瑟上皮剩余细胞作为对照染色。囊壁上皮巢细胞对CK13呈阳性反应，但其CK19染色与衬里上皮细胞中观察到的相似。钙化结节仅对CK13呈阳性反应。马拉瑟上皮剩余细胞对CK19呈阳性反应，但对CK13不呈阳性反应。仅在牙周膜纺锤状细胞核中观察到Cbfa-1阳性反应。CK13在棘细胞浅层呈阳性。CK19在口腔黏膜基底细胞中呈阳性。在囊肿的衬里上皮中，CK13和CK19的表达与其在口腔黏膜中的免疫反应相似。这些结果表明，牙源性上皮分化为鳞状上皮细胞，在COC中最初为影细胞，并且这一过程取决于分化的牙源性上皮细胞而非成骨细胞的营养不良性钙化。
