Huchet Aymeri, Belkacémi Yazid, Frick Johanna, Prat Marie, Muresan-Kloos Ioanna, Altan Dan, Chapel Alain, Gorin Norbert Claude, Gourmelon Patrick, Bertho Jean Marc
Service de Radiothérapie, Hôpital Européen G. Pompidou, Paris, France.
Int J Radiat Oncol Biol Phys. 2003 Oct 1;57(2):508-15. doi: 10.1016/s0360-3016(03)00584-4.
To determine whether variations in the plasma Flt-3 ligand (FL) concentration after radiotherapy (RT) may serve as a biomarker for radiation-induced bone marrow damage.
Twenty-seven patients were followed during RT. The irradiated bone marrow volume was determined. The blood cell counts and plasma FL concentrations were evaluated before and after RT. The expression of membrane-bound FL and mRNA expression were also defined in circulating blood cells.
We found a negative correlation between the plasma FL concentration and the number of circulating white blood cells and platelets during RT. Moreover, the overall amount of FL in the blood of patients during RT correlated directly with both the cumulated radiation dose and the proportion of irradiated bone marrow.
We demonstrated that the variations in plasma FL concentration directly reflect the radiation-induced bone marrow damage during fractionated local RT. We suggest a possible use for FL monitoring as a means to predict the occurrence of Grade 3-4 leukopenia or thrombocytopenia during the course of RT.
确定放疗（RT）后血浆Flt-3配体（FL）浓度的变化是否可作为辐射诱导骨髓损伤的生物标志物。
对27例患者在放疗期间进行随访。测定受照射的骨髓体积。评估放疗前后的血细胞计数和血浆FL浓度。还对循环血细胞中膜结合FL的表达和mRNA表达进行了定义。
我们发现放疗期间血浆FL浓度与循环白细胞和血小板数量呈负相关。此外，放疗期间患者血液中FL的总量与累积辐射剂量和受照射骨髓的比例均直接相关。
我们证明，在分次局部放疗期间，血浆FL浓度的变化直接反映了辐射诱导的骨髓损伤。我们建议FL监测可能作为一种手段，用于预测放疗过程中3-4级白细胞减少或血小板减少的发生。
