Prat Marie, Demarquay Christelle, Frick Johanna, Dudoignon Nicolas, Thierry Dominique, Bertho Jean Marc
Institut de Radioprotection et de Sûreté Nucléaire, DRPH/SRBE, Fontenay Aux Roses, France.
Radiat Res. 2006 Sep;166(3):504-11. doi: 10.1667/RR0568.1.
We evaluated the possibility of using plasma Flt3 ligand (FL) concentration as a biological indicator of bone marrow function after heterogeneous irradiation. Mice were irradiated with 4, 7.5 or 11 Gy with 25, 50, 75 or 100% of the bone marrow in the field of irradiation. This model of irradiation resulted in graded and controlled damage to the bone marrow. Mice exhibited a pancytopenia correlated with both the radiation dose and the percentage of bone marrow irradiated. The FL concentration in the blood increased with the severity of bone marrow aplasia. Nonlinear regression analysis showed that the FL concentration was strongly correlated with the total number of residual colony-forming cells 3 days after irradiation, allowing a precise estimate of residual hematopoiesis. Moreover, the FL concentration on day 3 postirradiation was correlated with the duration and severity of subsequent pancytopenia, suggesting that variations in FL concentrations might be used as a predictive indicator of bone marrow aplasia, especially by the use of linear regression equations describing these correlations. Our results provide a rationale for the use of FL concentration as a biological indicator of residual hematopoiesis after heterogeneous irradiation.
我们评估了将血浆Flt3配体（FL）浓度用作异质性照射后骨髓功能生物学指标的可能性。用4、7.5或11 Gy的剂量对小鼠进行照射，照射区域内的骨髓分别为25%、50%、75%或100%。这种照射模型导致骨髓出现分级且可控的损伤。小鼠表现出全血细胞减少，这与辐射剂量和照射的骨髓百分比均相关。血液中的FL浓度随着骨髓再生障碍的严重程度而增加。非线性回归分析表明，FL浓度与照射后3天残留集落形成细胞的总数密切相关，从而能够精确估计残留造血功能。此外，照射后第3天的FL浓度与随后全血细胞减少的持续时间和严重程度相关，这表明FL浓度的变化可能用作骨髓再生障碍的预测指标，特别是通过使用描述这些相关性的线性回归方程。我们的结果为将FL浓度用作异质性照射后残留造血功能的生物学指标提供了理论依据。
